No effect of napping on episodic foresight and prospective memory in kindergarten children.

Preschool children often have problems in remembering to carry out a planned behaviour. This study investigated the impact of napping on episodic foresight (planning for future events) and prospective memory (remembering to perform an action in the future) in 2-3-year-old children. In a quasi-experimental design, we compared children who napped (nap condition, n = 20) after receiving information about an upcoming problem (episodic foresight task) and a delayed intention (prospective memory task) with those who stayed awake (wake condition, n = 43).

Embodying anticipated affect enhances proactive behavior in 5-year-old children.

Imagining anticipated affects can foster future-oriented behavior in adults. However, children often still have difficulties in vividly imagining how they will feel in a specific episode (affective episodic future thinking [EFT]). We investigated whether enacting anticipated affects helps children to imagine how they will feel and whether this enhances proactive behavior in turn.

A conserved regulation of cell expansion underlies notochord mechanics, spine morphogenesis, and endochondral bone lengthening.

Cell size is a key contributor to tissue morphogenesis . As a notable example, growth plate hypertrophic chondrocytes use cellular biogenesis and disproportionate fluid uptake to expand 10-20 times in size to drive lengthening of endochondral bone . Similarly, notochordal cells expand to one of the largest cell types in the developing embryo to drive axial extension .

Correction: NGS read classification using AI.

[This corrects the article DOI: 10.1371/journal.pone.

Negative (but not positive) affective episodic future thinking enhances proactive behavior in 5-year-old children.

Envisioning the future and how you may feel (affective episodic future thinking [EFT]) helps adults to act in favor for their future self, according to manifold experiments. The current study tested whether and how affective EFT also helps children to behave more proactively, that is, to self-initially prepare for an upcoming event. Five-year-old ( = 90) children (data collected from 2021 to 2022) were instructed to mentally imagine how they would feel after successfully managing an upcoming test (positive affective EFT), how they would feel after failing to do so (negative affective EFT), or they were reminded of an upcoming test without a prompt to imagine (control condition, random assignment).

Physical and psychosocial factors are crucial for maintaining physical and mental health in endometriosis: a longitudinal analysis.

Objective: To test the associations of physical and psychosocial factors with physical and mental health in individuals living with endometriosis (EM) by means of cross-sectional and longitudinal analyses.

Methods And Measures: Data were gathered via an online survey between February and August 2021. At survey date t1, sociodemographic, EM-related and psychosocial factors as well as physical and mental health of people with EM were assessed.

[Psychosocial Care in the Intensive Care Unit].

The improvement of intensive care treatment options leads to an increasing number of patients being treated in this setting. For the majority of those affected and their relatives, this treatment is associated with tremendous stress, but also subsequent physical, psychological and cognitive impairments, the post-intensive care syndrome. The aim of psychosocial support in the intensive care unit is to stabilise and minimise the acute stress.

Future-oriented cognition: links to mental health problems and mental wellbeing in preschool-aged and primary-school-aged children.

Future-oriented cognition plays a manifold role for adults' mental health. The present study aimed to investigate the relationship between future-oriented cognition and mental health in  = 191 children aged between 3 and 7 years. Parents completed an online-questionnaire including children's future-oriented cognition (e.

Alternative ground covers and strip-tilling in CBD hemp production.

Objective: Little research has been done on managing soil health for large-scale, outdoor hemp production, contributing to the possible overuse of black plastic for weed suppression. Our experiment aimed to understand the performance of alternative ground covers including forage crops and hay as well as a less disruptive tilling method called strip-tilling compared to black plastic.

Results: Yield and soil health data were taken from three experimental plantings from two different outdoor CBD hemp farms in Vermont, USA.

The Prenucleation Equilibrium of the Parathyroid Hormone Determines the Critical Aggregation Concentration and Amyloid Fibril Nucleation.

Nucleation and growth of amyloid fibrils were found to only occur in supersaturated solutions above a critical concentration (c ). The biophysical meaning of c remained mostly obscure, since typical low values of c in the sub-μM range hamper investigations of potential oligomeric states and their structure. Here, we investigate the parathyroid hormone PTH as an example of a functional amyloid fibril forming peptide with a comparably high c of 67±21 μM.

Differences and similarities in personality functioning across different types of eating disorders.

Objective: The classification of anorexia nervosa (AN) into subtypes is relevant due to their different symptomatology. However, subtypes (restricting type: AN-R; purging type: AN-P) differ also in terms of their personality functioning. Knowledge about these differences would allow for better treatment stratification.

Controlled -Type Doping of Pyrite FeS.

Pyrite FeS has extraordinary potential as a low-cost, nontoxic, sustainable photovoltaic but has underperformed dramatically in prior solar cells. The latter devices focus on heterojunction designs, which are now understood to suffer from problems associated with FeS surfaces. Simpler homojunction cells thus become appealing but have not been fabricated due to the historical inability to understand and control doping in pyrite.

Inherent Adaptivity of Alzheimer Peptides to Crowded Environments.

Amyloid β (Aβ) is the major constituent in senile plaques of Alzheimer's disease in which peptides initially undergo structural conversions to form elongated fibrils. The impact of crowding on the fibrillation pathways of Aβ and Aβ , the most common peptide isoforms are studied. PEG and Ficoll are used as model crowders to mimic a macromolecular enriched surrounding.

Investigation of semi- and self-supervised learning methods in the histopathological domain.

Training models with semi- or self-supervised learning methods is one way to reduce annotation effort since they rely on unlabeled or sparsely labeled datasets. Such approaches are particularly promising for domains with a time-consuming annotation process requiring specialized expertise and where high-quality labeled machine learning datasets are scarce, like in computational pathology. Even though some of these methods have been used in the histopathological domain, there is, so far, no comprehensive study comparing different approaches.

EU regulation of gene-edited plants-A reform proposal.

This article presents a proposal on how the European Union's regulatory framework on genetically modified (GM) plants should be reformed in light of recent developments in genomic plant breeding techniques. The reform involves a three-tier system reflecting the genetic changes and resulting traits of GM plants. The article is intended to contribute to the ongoing debate over how best to regulate plant gene editing techniques in the EU.

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish.

The advent of targeted CRISPR-Cas nuclease technologies has revolutionized the ability to perform precise genome editing in both established and emerging model systems. CRISPR-Cas genome editing systems use a synthetic guide RNA (sgRNA) to target a CRISPR-associated (Cas) endonuclease to specific genomic DNA loci, where the Cas endonuclease generates a double-strand break. The repair of double-strand breaks by intrinsic error-prone mechanisms leads to insertions and/or deletions, disrupting the locus.

Human endothelial cells form an endothelium in freestanding collagen hollow filaments fabricated by direct extrusion printing.

Fiber-shaped materials have great potential for tissue engineering applications as they provide structural support and spatial patterns within a three-dimensional construct. Here we demonstrate the fabrication of mechanically stable, meter-long collagen hollow filaments by a direct extrusion printing process. The fibres are permeable for oxygen and proteins and allow cultivation of primary human endothelial cells (ECs) at the inner surface under perfused conditions.

A Competition of Secondary and Primary Nucleation Controls Amyloid Fibril Formation of the Parathyroid Hormone.

Functional amyloids belong to an increasing class of non-toxic biologic material, in contrast to the prominent disease-related amyloids. Herein, this work reports on the fibril formation of the parathyroid hormone PTH as a representative candidate following the same generic principles of primary and secondary nucleation. Employing Thioflavin T monitored kinetics analyses and negative-staining transmission electron microscopy, an intricate, concentration dependent behavior of time dependent generation and morphologies of PTH fibrils are found.

The pro-sequence of parathyroid hormone prevents premature amyloid fibril formation.

The parathyroid hormone (PTH) regulates the calcium and phosphate level in blood after secretion from parathyroid chief cells. The pre- and pro-sequences of precursor preproPTH get cleaved during PTH maturation. In secretory granules, PTH forms functional amyloids.

A Path Toward Equity and Inclusion: Establishing a DEI Committee in a Department of Pediatrics.

The Diversity, Equity, and Inclusion (DEI) committee was established in 2017 within the Department of Pediatrics at Rush University Medical Center (RUMC), an academic medical health center located on the near west side of Chicago, IL. Results from climate surveys highlighted the need for increased DEI initiatives within the department, and a renewed national reckoning on racial tensions sparked an additional sense of urgency for system-level change. This paper outlines the initial creation and ongoing efforts of the DEI committee.

Heparin promotes rapid fibrillation of the basic parathyroid hormone at physiological pH.

In acidic secretory granules of mammalian cells, peptide hormones including the parathyroid hormone are presumably stored in the form of functional amyloid fibrils. Mature PTH, however, is considerably positively charged in acidic environments, a condition known to impede unassisted self-aggregation into fibrils. Here, we studied the role of the polyanion heparin on promoting fibril formation of PTH.

Modulating the Fibrillization of Parathyroid-Hormone (PTH) Peptides: Azo-Switches as Reversible and Catalytic Entities.

We here report a novel strategy to control the bioavailability of the fibrillizing parathyroid hormone (PTH)-derived peptides, where the concentration of the bioactive form is controlled by an reversible, photoswitchable peptide. PTH, a human hormone secreted by the parathyroid glands, is important for the maintenance of extracellular fluid calcium and phosphorus homeostasis. Controlling fibrillization of PTH represents an important approach for in vivo applications, in view of the pharmaceutical applications for this protein.

Lighting up Nobel Prize-winning studies with protein intrinsic disorder.

Intrinsically disordered proteins and regions (IDPs and IDRs) and their importance in biology are becoming increasingly recognized in biology, biochemistry, molecular biology and chemistry textbooks, as well as in current protein science and structural biology curricula. We argue that the sequence → dynamic conformational ensemble → function principle is of equal importance as the classical sequence → structure → function paradigm. To highlight this point, we describe the IDPs and/or IDRs behind the discoveries associated with 17 Nobel Prizes, 11 in Physiology or Medicine and 6 in Chemistry.

Chronic Pelvic Pain in Endometriosis: Cross-Sectional Associations with Mental Disorders, Sexual Dysfunctions and Childhood Maltreatment.

The aim of this cross-sectional study was to compare the rates of mental disorders, sexual dysfunctions and childhood maltreatment (CM) in women with endometriosis with either chronic pelvic pain (CPP) or minimal to no pelvic pain. Additionally, two models to predict a current mental disorder were tested, including pelvic-pain-related or psychosocial predictor variables. We examined 100 women with confirmed endometriosis (group CPP, n = 50; group NOPAIN, n = 50).