[Modification of experimental rotenone model of Parkinson's disease].

A modification of experimental model of Parkinson's disease is proposed presuming the stereotaxic infusion of rotenone solution using a special device into the cental part of substantia nigra (SN) pars compacta of adult Wistar rats. It was shown that 10 days after infusion of the neurotoxin the density of dopaminergic (DA) neurons in the infusion area drops nearly six-fold, to 20.2±3.

Xenotransplantation of cultured newborn pig thyroid tissue for the treatment of post-radioiodine hypothyroidism in rats.

The aim of the present study was to develop atransplantation technique for restoration of thyroidfunction in rats with radioiodine-inducedhypothyroidism. Each Wistar rat received the dose of75.0 muCi of 131-iodine by intraperitonealinjection.

The beta-subunit composition of nicotinic acetylcholine receptors in the neurons of the guinea pig inferior mesenteric ganglion.

The antibodies against synthetic (183-192) fragments of beta2- and beta4-subunits of rat neuronal nicotinic acetylcholine receptor were used to study a beta-subunit composition of nicotinic receptors in the inferior mesenteric ganglion of the guinea pig by both immunocytochemical staining and blocking of excitatory postsynaptic potentials induced by electric stimulation of the pre-ganglionic nerve (intermesenteric trunk). The beta4-specific antibody stained 59.8 +/- 7.

Nicotinic acetylcholine receptor subtypes in rat superior cervical ganglion neurons as studied by sequential application of two alpha-subunit-specific antibodies.

All cultured neurons of rat superior cervical ganglion (SCG) were stained with nicotinic acetylcholine receptor (nAChR) alpha5- or alpha7-subunit-specific oligoclonal antibodies (Abs) and could additionally bind alpha3-subunit-specific monoclonal antibody (mAb). About 60% of the neurons were stained with alpha4-specific Ab and could not bind alpha3-specific mAb. The acetylcholine-induced membrane currents recorded with the whole-cell patch clamp method and partially blocked with alpha3-specific mAbs, could be additionally blocked with alpha5- and alpha7- specific Abs, and vice versa.

Alpha subunit composition of nicotinic acetylcholine receptors in the rat autonomic ganglia neurons as determined with subunit-specific anti-alpha(181-192) peptide antibodies.

The subunit composition of nicotinic acetylcholine receptors of rat autonomic ganglia neurons was studied by means of antibodies, which differentiated between different alpha subunits and specifically blocked acetylcholine-induced membrane currents. Polyclonal rabbit antibodies and mouse monoclonal antibodies were raised against synthetic peptides matching in sequence the alpha(181-192) region of alpha3, alpha4, alpha5, and alpha7 subunits of rat neuronal nicotinic acetylcholine receptors. The antibodies discriminated among alpha3, alpha4, alpha5, and alpha7 peptides in enzyme-linked immunosorbent assay and bound to native acetylcholine receptors expressed in PC-12 cells.

Participation of the medial reticular formation of the medulla oblongata in the supraspinal control of locomotor and postural activities in the guinea pig.

The influence of microinjections of novocaine (1.5 microliters of a 2% solution) into the ventromedial areas of the reticular formation of the medulla oblongata on the effects of electrical stimulation of the mesencephalic locomotor region, and of adequate stimulation of the vestibular apparatus, was investigated in experiments on decerebrate guinea pigs. It was demonstrated that such injections are accompanied by a reversible increase in the threshold of stimulation of the region indicated, necessary for the initiation of locomotor rhythmicity, as well as by the suppression of vestibular influences on the activity of the flexor muscles of the extremities.

[Organization of vestibulospinal systems in vertebrates].

The data about vestibular nuclear complex and vestibulospinal systems in the submammalian and mammalian vertebrates are offered in the review. Information presented in the world literature concerning the vestibular nuclei topography, structure, fibre connections and influences on the spinal cord and skeletal muscles is summarized. The current conceptions of the structural and functional specificity of different vestibulospinal systems are considered.

[The participation of the medial reticular formation of the medulla oblongata in the supraspinal control of locomotor and postural activities in the guinea pig].

The effect of procain microinjection into the ventromedial part of medullary RF on the effects of electrical stimulation of mesencephalic locomotor region (MLR) and vestibular adequate stimulation was investigated in the decerebrated guinea pig. The injection was followed by reversible increasing of MLR stimulation threshold necessary for the initiation of locomotion and by the decreasing of vestibular influence on forelimb flexor activity. The participation of RF in the supraspinal control of posture and locomotion in discussed.

[Preganglionic parasympathetic innervation of the intact and partially denervated rat stomach].

The preganglionic parasympathetic innervation of normal and vagotomized rat stomach have been investigated by the horseradish peroxidase (HRP) technique. After the HRP injection into the antrum and fundus muscles the labelled cells were revealed in nuclei dorsalis nervi vagi bilaterally, but preferentially in the left nucleus. The most numerous labelled cells were observed after injection into the region of the curvatura minor.

[Comparative analysis of the dimensions of vestibulo-spinal neurons in the guinea pig].

The comparative analysis of dimensions of horseradish peroxidase labelled vestibulo-spinal neurons and neutral red stained neurons of undifferentiated areas of the vestibular nuclei have been performed in guinea pig. The undifferentiated areas of the vestibular nuclei consisted of small, medium, large and giant cells. A decrease in the relative numbers of small- and medium-diameter neurons and an increase in the relative numbers of large and giant neurons have been observed along the caudo-rostral axis of the vestibular complex.

The effect of procaine injection into the medullary reticular formation on forelimb muscle activity evoked by mesencephalic locomotor region and vestibular stimulation in the decerebrated guinea-pig.

The effect of procaine microinjection into the ventromedial portion of the medullary reticular formation on forelimb muscle activity evoked by electrical mesencephalic locomotor region and natural vestibular stimulation has been investigated in the decerebrated guinea-pig. This injection is followed by a reversible increase of the threshold of mesencephalic locomotor region stimulation necessary for the activation of muscle rhythmic activity. Procaine injection is accompanied by reduction of vestibular influence on flexor muscle activity evoked by electrical cutaneous and mesencephalic locomotor region stimulation.

[The spatial organization of the vestibulospinal neurons in the guinea pig].

Quantitative investigation of the spatial organization of vestibulospinal neurons has been performed on a guinea pig using the horseradish peroxidase retrograde transport method. After unilateral injection in the upper cervical and low thoracic spinal cord the labelled neurons were found unilaterally in the lateral vestibular nucleus and bilaterally in descending and medial vestibular nuclei. Distribution of vestibulospinal neurons within the brainstem has been analyzed.

[The vestibular nuclei of the guinea pig: their structural and topical organization].

Topographical and cytoarchitectonical organization of the vestibular nuclear complex of guinea pig has been studied. Four vestibular nuclei: superior, lateral, medial, inferior as well as cellular groups (I, F, Y, X, Z) associated with the vestibular nuclei were described on the basis of neutral red stained preparations. The precise delimitation and the stereotaxic coordinates of the vestibular nuclei were performed by means of the analysis of 50 microns thick frontal serial slices treated by glycerine.

[Vestibulofugal projections to the lateral reticular nucleus of the cat medulla].

Certain amount of neurons in vestibular nuclei were labelled after horseradish peroxidase injection in the medullar lateral reticular nucleus of cat. Such projections were strictly homolateral and formed by small and medium-size neurons localized mainly in the Deiters nucleus; their number was, probably, much less in comparison with the number of vestibulo-spinal units. Functional role of vestibular projections to the lateral reticular nucleus in the motor activity control is discussed.

[Spatial organization of the sources of the spino-reticulo-cerebellar pathway].

Quantitative characteristics of spatial distribution of HRP-labelled neurons projecting to the medullar lateral reticular nucleus (LRN) were calculated in the cat spinal cord. Mean quantities of the labelled units in the transverse 60 micron sections were 12.55 at C2-C4, 7.

[Dynamic properties of postural reflexes of the neck in the cat].

Changes in the forelimb extensor reflexes during fixed shifts and cyclic tilting of the head were investigated in decerebrated vestibuloectomized cats. In the used frequency range (0.05-1.

[Comparative dimensions of propriospinal neurons of different structuro-functional groups in the cat].

Profile areas of somata and proximal dendrites and conventional diameters (c.d.--diameter of a circle equiareal to this profile) of descending propriospinal interneurons labelled by horseradish peroxidase were measured in cat spinal cord.

[Changes in the electric activity of the rat stomach during direct roentgen irradiation].

In experiments on rats inhibition of electric activity of X-irradiated stomach was discovered. The disturbance of the nervous regulation of the exposed stomach was manifested mainly by inhibition of the transmission of exciting stimuli via choline- and serotonin--reactive structures of the vegetative nervous system. The administration of serotonin and phentolamine normalized the stomach activity.

[Contact microscopic and histochemical studies of the placenta in normal and pathological pregnancy].

The placenta was studied in 70 women: 19 of them had physiological pregnancy and 51 had pregnancy complicated with late toxicosis. Lesions in the placenta have been shown to increase with the growing severity of toxicosis, and compensatory potencies--to decrease. In late toxicosis the expansion of desorganization and reparation in the placenta occurs from the center towards periphery, the peripheral parts playing the role of reserved zones of the placenta, keeping high functional activity.