Publications by authors named "Vlasta Jencic"

Article Synopsis
  • Euthanasia practices for zebrafish larvae younger than 5 days post fertilization are not well-documented, and standardized guidelines are lacking due to legal protections not covering these early-stage fish.
  • The study evaluated various euthanasia methods, including anesthetic overdoses and hypothermic shock, showing that clove oil, lidocaine with ethanol, and electrical stunning were the most effective with minimal signs of distress.
  • The recommended euthanasia method is a buffered lidocaine hydrochloride solution with ethanol, which effectively euthanizes embryos and larvae while minimizing stress; further research is needed for electrical stunning's efficacy.
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The aim of this preliminary research was to establish if there are intersex occurrences in wild freshwater fish in Slovenian rivers and streams. In the first study we evaluated all fish species of both sexes obtained from the river Ljubljanica from its source to mouth. In the second study we focused on the rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta m.

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The name 'Mycobacterium angelicum' dates back to 2003 when it was suggested for a slowly growing mycobacterium isolated from freshwater angelfish. This name is revived here and the novel species is proposed on the basis of the polyphasic characterization of four strains including the original one. The four strains presented 100 % 16S rRNA gene sequence similarity with Mycobacterium szulgai but clearly differed from M.

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All 5 crayfish species inhabiting Slovenian freshwaters, of which 3 are indigenous crayfish species (ICS: Astacus astacus, Austropotamobius pallipes, and A. torrentium) and 2 are non-indigenous (NICS: Pacifastacus leniusculus and Cherax quadricarinatus), were inspected for the presence of Aphanomyces astaci, the causative agent of crayfish plague. Wild crayfish populations showing no clinical signs of infection were inspected using A.

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In November and December 2007, the virus causing viral haemorrhagic septicaemia (VHS) was detected in rainbow trout Oncorhynchus mykiss from 2 fish farms in Slovenia. During 2008 and 2009 the infection spread only among rainbow trout farms and 4 new outbreaks were confirmed. High mortality and clinical signs of VHS were observed among the diseased fish.

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The effect of abamectin (ABM) on rainbow trout (Oncorhynchus mykiss) was studied. The acute toxicity of ABM on rainbow trout was established, following the target 58-h water bath exposure of ABM concentrations from 0.6 to 4.

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The detection of infectious hematopoietic necrosis virus (IHNV) in infected rainbow trout Oncorhynchus mykiss and in cell culture supernatants stored under different conditions was studied. IHNV-positive fish visceral organ homogenates and cell culture supernatants were incubated at 4 and 25 degrees C. Virus titre was measured by virus isolation on epithelioma papulosum cyprini (EPC) cells and the IHNV RNA was detected by RT-PCR and semi-nested RT-PCR.

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