[Kinetics of the interaction of ferrous oxide with oxidized lipids and possibility of chiluminescent determination of hydroperoxides].

The theory shows that for lipids containing hydroperoxides of unsaturated fatty acids there exists a verge concentration of Fe2+: at concentrations of Fe2+ lower than the verge one the peroxidation process develops autocatalytically and at higher concentrations it looks pulseshaped. In the second case the amplitude of pulse concentration of peroxide radicals and the square root from the maximum value of chemiluminescence intensity are proportional to the concentration of hydroperoxides present in the system at the time of ferrous ions injection. The former two values do not depend on the concentration of inoxidized fatty acids.

[Relation between fluorescence and circular dichroism of the complex of the fluorescence probe 4-dimethylaminochalcone with serum albumin].

The fluorescence probe(4-dimethylaminochalcone; DMH) was noncovalently linked to human serum albumin (HSA). The variation of pH was due to serum albumin structural changes, which was determined in terms of DMH and HSA fluorescence and CD spectra. Considerable changes of fluorescence and CD spectra were observed at pH 8 and 10, where there is ionization of two more recently titrated tyrosin residues.

[Fluorimetric study of phospholipase A-induced efflux of Ca2+ from liposomes].

Artificial vesicles, liposomes, were prepared from the total fraction of phospholipids of rat liver mitochondria. Electron microscopy showed that the structure of liposomes depended on cation composition of the medium in which they were formed. Fluorescence of chlorotetracycline increased in the suspension of liposomes loaded with Ca+2 due to the formation of Ca+2-chlorotetracycline-phospholipid membrane complex.

[Letter: Effect of lipid peroxidation on the permeability of liposome membranes for Ca2+ ions].

Efflux of Ca2+ ions from liposomes after peroxidation of their lipids was studied using fluorescent assay with chlorotetracicline. It was shown that products of lipid peroxidation disturbed the stability of liposome membranes and increased their permeability for Ca2+ ions.