Remodeling of the chromatin landscape in peripheral blood cells in patients with severe Delta COVID-19.

COVID-19 is characterized by systemic pro-inflammatory shifts with the development of serious alterations in the functioning of the immune system. Investigations of the gene expression changes accompanying the infection state provide insight into the molecular and cellular processes depending on the sickness severity and virus variants. Severe Delta COVID-19 has been characterized by the appearance of a monocyte subset enriched for proinflammatory gene expression signatures and a shift in ligand-receptor interactions.

A Transverse Hamiltonian Approach to Infinitesimal Perturbation Analysis of Quantum Stochastic Systems.

This paper is concerned with variational methods for open quantum systems with Markovian dynamics governed by Hudson-Parthasarathy quantum stochastic differential equations. These QSDEs are driven by quantum Wiener processes of the external bosonic fields and are specified by the system Hamiltonian and system-field coupling operators. We consider the system response to perturbations in these operators and introduce a transverse Hamiltonian which encodes the propagation of the perturbations through the unitary system-field evolution.

Functional Characteristics of Serum Anti-SARS-CoV-2 Antibodies against Delta and Omicron Variants after Vaccination with Sputnik V.

Anti-SARS-CoV-2 vaccination leads to the production of neutralizing as well as non-neutralizing antibodies. In the current study, we investigated the temporal dynamics of both sides of immunity after vaccination with two doses of Sputnik V against SARS-CoV-2 variants Wuhan-Hu-1 SARS-CoV-2 G614-variant (D614G), B.1.

A 5-year multicentre randomized controlled trial comparing personalized, frozen and fresh blastocyst transfer in IVF.

Research Question: Does clinical performance of personalized embryo transfer (PET) guided by endometrial receptivity analysis (ERA) differ from frozen embryo transfer (FET) or fresh embryo transfer in infertile patients undergoing IVF?

Design: Multicentre, open-label randomized controlled trial; 458 patients aged 37 years or younger undergoing IVF with blastocyst transfer at first appointment were randomized to PET guided by ERA, FET or fresh embryo transfer in 16 reproductive clinics.

Results: Clinical outcomes by intention-to-treat analysis were comparable, but cumulative pregnancy rate was significantly higher in the PET (93.6%) compared with FET (79.

How Working Memory Provides Representational Change During Insight Problem Solving.

Numerous studies of insight problem solving are focused on both the control and storage systems of working memory. We obtained contradictory data about how working memory systems are involved in insight problem solving process. We argue that measuring the dynamics of the control system and storage systems through the course of problem solving can provide a more refined view on the processes involved, as a whole, and explain the existing controversies.

Energy barriers at grain boundaries dominate charge carrier transport in an electron-conductive organic semiconductor.

Semiconducting organic films that are at the heart of light-emitting diodes, solar cells and transistors frequently contain a large number of morphological defects, most prominently at the interconnects between crystalline regions. These grain boundaries can dominate the overall (opto-)electronic properties of the entire device and their exact morphological and energetic nature is still under current debate. Here, we explore in detail the energetics at the grain boundaries of a novel electron conductive perylene diimide thin film.

Theory about the Embryo Cryo-Treatment.

Background: To create hypothesis, which can give a logical explanation related to the benefits of freezing/thawing embryos. Cryopreservation is not only a technology used for storing embryos, but also a method of embryo treatment that can potentially improve the success rate in infertile couples.

Methods: From the analysis of multiple results in assisted reproductive technology, which have no satisfactory explanation to date, we found evidence to support a 'therapeutic' effect of the freezing/thawing of embryos on the process of recovery of the embryo and its subsequent implantation.

High-Mobility, Ultrathin Organic Semiconducting Films Realized by Surface-Mediated Crystallization.

The functionality of common organic semiconductor materials is determined by their chemical structure and crystal modification. While the former can be fine-tuned via synthesis, a priori control over the crystal structure has remained elusive. We show that the surface tension is the main driver for the plate-like crystallization of a novel small organic molecule n-type semiconductor at the liquid-air interface.

[Opine biosynthesis and catabolism genes of Agrobacterium tumefaciens and Agrobacterium rhizogenes].

Agrobacterium is a genus of soil bacteria with the ability to transform plant cells by a T-DNA-sequence located on the pTi/pRi- plasmid containing a set of genes expressed in plant cells. Expression of these genes leads to a proliferation of transformed cells, with the subsequent formation of tumors or growths of roots and the synthesis of opines--products of the condensation of amino acids with ketoacids or sugars used by Agrobacteria as a source of carbon and nitrogen. In this review, we systematized the information about most common opines in plant--Agrobacterium systems and their biosynthesis and catabolism genes, as well as the role of opines in the interaction of pathogenic Agrobacterium with plants and with other Agrobacterium strains, including the genetic consequences of such interactions.

[Genetics of anthocyaninless rye].

Six nonallelic genes have been discovered in rye, the recessive mutations of which lead to a lack of anthocyanin. Crosses with these mutants showed that 13 new anthocyaninless lines carry mutations in the gene vi1, whereas vi2/6 mutations were identified only in single cases. Inheritance of the vi1/6 mutations in the progeny of hybrids with the wild type (Line 7, L7) corresponds to a monohybrid segregation.

Fibulin-5 (FBLN5) gene polymorphism is associated with pelvic organ prolapse.

Objective: FBLN5 encodes a key protein of elastic fiber matrix assembly and function that contributes to maintaining pelvic support and plays the important role in the pathophysiology of pelvic organ prolapse (POP). The aim of the study was to investigate whether there is an association between common single-nucleotide polymorphisms (SNPs) of the FBLN5 gene and POP.

Study Design: A total of eleven tag SNPs of the FBLN5 gene were genotyped using the polymerase chain reaction with confronting two-pair primers (PCR-CTPP) in 210 patients with POP (stages III-IV) and 292 controls with no even minimal POP.

[Effects of low power laser radiation of blue, green and red ranges on free radical processes in rat blood in endotoxic shock].

This study was performed to investigate the effects of low power laser radiation in blue (441.2 nm), green (532.5 nm) and red (632.

[The role of tetrapyrrol photosensitizers in photoinduced variation of free radical characteristics of rat blood in endotoxic shock].

The main goal of the present study was to evaluate the comparative effectiveness of tetrapyrrol photosensitizers (protoporphyrine IX and chlorine e6) in red (632.8 nm) and green (532.5) spectrum bands on rat blood free radical status, using the experimental model of endotoxic shock.

[Kinetic chemiluminescence as a method for the study of free radical reactions].

The review gives concepts of analysis of kinetics of complex reactions with the participation of free-radicals. The concepts are based on the comparison of the kinetic curves of the chemiluminescent reactions in the presence of a physical enhancer coumarin C-525 with the simulated kinetic functions. This method was applied to the investigation of the mechanism of a branched-chain reaction of lipid peroxidation in biological and phospholipid membranes as well as to the effect of antioxidants and determination of their activities.

[Role of endogenous porphyrins in the effects of low-intensity laser radiation of the red region on free radical processes in the blood of rats under experimental endotoxic shock].

The role of endogenous porphyrins in the effects of laser radiation of the red region (632.8 nm) on free radical processes in the blood of rats under endotoxic shock induced by the administration of lipopolysaccharide B (25 mg/kg) has been studied. The measurements of the functional activity of polymorphonuclear leukocytes (the method of luminol-dependent chemiluminescence), the superoxide dismutase activity of blood plasma (using nitro blue tetrazolium), and the degree of lipid oxidation of erythrocyte membranes (the method of fluorescence of cis-parinaric acid) have been carried out.

[Antioxidants as aromatic amino acid oxidation products].

The accumulation of UV photolysis products of amino acids tyrosine and tryptophan, which possess an antioxidant activity, has been studied by the method of luminol-activated chemiluminescence. The amount of antioxidant products was judged by the value of the total antioxidant potential of a UV-irradiated solution, the measure of which was the distance between the peaks of the chemiluminescence curve in the system 2,2'-azo-bis(2-amidinopropane)hydrochloride + luminol in a UV-irradiated and an unirradiated samples (induction period, tau(i)). Simultaneously, the absorption and fluorescence spectra of unirradiared and UV-irradiated amino acid solutions were recorded.

[Role of endogenous porphyrins in laser therapy of experimental skin wounds].

The role of endogenous porphyrins in the effect of laser irradiation on the superoxide dismutase (SOD) activity of wound exudate and rat leukocyte activity has been studied on models of aceptic incised skin wounds. Wounds were irradiated by a He-Ne laser (632.8 nm, 1.

[First live birth in Bulgaria after in vitro maturation (IVM) procedure (case report)].

The objective is to report the first healthy live birth in Bulgaria from immature oocytes retrieved in a natural menstrual cycle, followed by in vitro maturation (IVM). In vitro maturation protocol was performed in a case with male factor of infertility. Twentyone immature oocytes were retrieved by transvaginal ultrasound guided follicle aspiration on day 10 of a 28-year-old woman and matured in vitro.

[Production of reactive oxygen species by monocyte-derived macrophages from the blood of healthy donors and patients with IHD].

Production of reactive oxygen species (ROS) by macrophages from blood monocytes of healthy donors (MP(N)) and patients with IHD (MP(IHD)) before, during, and after their incubation with low-density lipoprotein (LDL) isolated from the blood plasma of healthy donors (LDL(N)) and patients with a high cholesterol level (LDL(H)) was estimated by the method of luminol-dependent and stimulated by opsonized zymosan (OZ) or phorbol-12-myristate-13-acetate (PMA) chemiluminescence (CL). Intrinsic luminol-dependent, and zymosan- or PMA-stimulated chemiluminescence of MP(IHD) have exceeded the same types of chemiluminescence of MP(N) by factors of 1.4, 1.

[A study of the effect of low-intensity laser radiation of the blue, green, and red spectral regions on the healing of experimental skin wounds in rats].

The effect of low-intensity laser radiation of the blue (441.2 nm), green (532 nm), and red (632.8 nm) spectral regions on the healing of experimental skin wounds in rats has been studied.

[Endocrinology of in vitro maturation of oocytes].

In-vitro maturation (IVM) of oocytes is an alternative to conventional in-vitro fertilization (IVF) treatment. Significant progress has been made to improve pregnancy and implantation rates from in-vitro matured oocytes. However oocyte maturation in vitro is profoundly affected by culture conditions.

[Lucigenin-enhanced chemiluminescence of the animal tissues].

Lucigenin-enhanced chemiluminescence (LcCL) allows one to investigate the reactions of superoxide anion radical (*O2-) generated by mitochondria and is applied to study the superoxide production in enzymatic and membrane systems by isolated mitochondria and cells, and in whole organs. The application of lucigenin-enhanced chemiluminescence to estimate the respiration of human tissues involves the use of small tissue pieces, which can be obtained, for instance, by biopsia; however, no systematic investigations have been performed on these objects. In the present paper, a comparative study of lucigenin-enhanced chemiluminescence of tissues isolated from different organs of the rat was carried out to elucidate its dependence on the extent of tissue defragmentation, storage time, and access for oxygen.

[Radiation methods for monitoring parameters of X-ray diagnostic apparatuses].

Capabilities of noninvasive dosimeters for radiation monitoring of parameters of X-ray diagnostic apparatuses are considered. It is shown that monitoring of the half-value layer using the Unfors-Xi detector makes it possible to correct the results of radiation contrast measurement taking into account the actual (measured) values of half-value layers. It also simplifies the procedure for half-value layer monitoring in radiation safety measurements.

Edge-to-site reduction of Bethe-Peierls approximation for nearest neighbor exclusion cubic lattice particle systems and thermodynamic modeling of liquid silicates.

We study an interacting particle system on the simple cubic lattice satisfying the nearest neighbor exclusion (NNE) which forbids any two nearest sites to be simultaneously occupied. Under the constraint, we develop an edge-to-site reduction of the Bethe-Peierls entropy approximation of the cluster variation method. The resulting NNE-corrected Bragg-Williams approximation is applied to statistical mechanical modeling of a liquid silicate formed by silica and a univalent network modifier, for which we derive the molar Gibbs energy of mixing and enthalpy of mixing and compare the predictions with available thermodynamic data.