Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus.

In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus.

The use of electroporation to deliver DNA-based vaccines.

Introduction: Nucleic acids represent a promising platform for creating vaccines. One disadvantage of this approach is its relatively low immunogenicity. Electroporation (EP) is an effective way to increase the DNA vaccines immunogenicity.

In situ phase formation during high-temperature synthesis in clad mechanocomposites based on the Ti-Al system.

An in situ synchrotron experimental study of phase formation dynamics in clad mechanocomposites of Ti-Al systems during high-temperature synthesis was performed. Cladding of the obtained mechanocomposites was carried out with an SiO target, with a deposition time of 40 min. The high-temperature synthesis was performed using the thermal explosion method based on a microwave induction heater in the in situ mode on an experimental setup adapted to synchrotron radiation time-resolved diffractometry.

Author Correction: Chronicles of nature calendar, a long-term and large-scale multitaxon database on phenology.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Chronicles of nature calendar, a long-term and large-scale multitaxon database on phenology.

We present an extensive, large-scale, long-term and multitaxon database on phenological and climatic variation, involving 506,186 observation dates acquired in 471 localities in Russian Federation, Ukraine, Uzbekistan, Belarus and Kyrgyzstan. The data cover the period 1890-2018, with 96% of the data being from 1960 onwards. The database is rich in plants, birds and climatic events, but also includes insects, amphibians, reptiles and fungi.

Synchrotron in situ studies of mechanical activation treatment and γ-radiation impact on structural-phase transitions and high-temperature synthesis parameters during the formation of γ-(TiAl) compound.

In situ synchrotron studies of structure and phase formation dynamics in mechanically activated (t = 7 min, power density 40 g) and mechanically activated with subsequent irradiation by γ-quanta Co powder mixture (Ti 64 wt% + Al) during high-temperature synthesis by the method of thermal explosion using induction heating are described. In situ high-temperature synthesis was carried out on the created experimental complex adapted for synchrotron X-ray diffraction methods. The sequence of formation and time-temperature interval of the metastable and main phases were determined.

In situ synchrotron research of phase formation in mechanically activated 3Ti + Al powder composition during high-temperature synthesis under the condition of heating with high-frequency electromagnetic fields.

An in situ synchrotron study of the specific features of the phase formation dynamics in mechanically activated 16 wt% Al + Ti powder composition is described, the high-temperature synthesis being carried out under the condition of high volume inflammation by means of inductive heating. The kinetics of the phase formation were registered with an experimental complex, especially designed, constructed and adjusted for the method of dynamic diffraction analysis in synchrotron radiation beams. It has been experimentally in situ shown that increasing the time of mechanical activation of the initial powder mixture reduces the temperature at which components start to react and the time of realization of the high-temperature synthesis.

Variation of Reproductive Traits and Female Body Size in the Most Widely-Ranging Terrestrial Reptile: Testing the Effects of Reproductive Mode, Lineage, and Climate.

The European common lizard, , is the most widespread terrestrial reptile in the world. It occupies almost the entire Northern Eurasia and includes four viviparous and two oviparous lineages. We analysed how female snout-vent length (SVL), clutch size (CS), hatchling mass (HM), and relative clutch mass (RCM) is associated with the reproductive mode and climate throughout the species range and across the evolutionary lineages within .