Publications by authors named "Vladimir Wischnewski"
Brain metastases are the most common brain tumors in patients and are associated with poor prognosis. Investigating the colonization and outgrowth of brain metastases is challenging given the complexity of the organ, tissue sampling difficulty, and limited experimental models. To address this challenge, we employed a strategy to analyze the metastatic niche in established lesions, based on the release of a cell-penetrating mCherry tag from labeled tumor cells to neighboring niche cells, using different brain metastasis mouse models.
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- Melanoma often spreads to the brain, making it crucial to understand how melanoma cells pass through the blood-brain barrier (BBB) to prevent brain metastases.
- Using primary mouse brain microvascular endothelial cells (pMBMECs), researchers studied how melanoma cells interact with and disrupt the BBB, noting that inhibiting certain proteases can help restore this barrier.
- The study also involved a new melanoma cell line and experiments with PECAM-1-deficient mice, showing that compromised barrier properties lead to increased melanoma cell movement across the BBB, highlighting the importance of maintaining BBB integrity to reduce brain metastasis.
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- * A study looked closely at neutrophils in tumor tissues from patients with glioma (a type of brain cancer) and brain metastasis (cancer that has spread to the brain) and compared them to those in blood.
- * The researchers found that neutrophils in brain tumors are different from those in blood; they live longer and can help tumors grow by suppressing the immune response and causing inflammation.
The immune-specialized environment of the healthy brain is tightly regulated to prevent excessive neuroinflammation. However, after cancer development, a tissue-specific conflict between brain-preserving immune suppression and tumor-directed immune activation may ensue. To interrogate potential roles of T cells in this process, we profiled these cells from individuals with primary or metastatic brain cancers via integrated analyses on the single-cell and bulk population levels.
View Article and Find Full Text PDFHigh-grade gliomas, the most common and aggressive primary brain tumors, are characterized by a complex tumor microenvironment (TME). Among the immune cells infiltrating the glioma TME, tumor-associated microglia and macrophages (TAMs) constitute the major compartment. In patients with gliomas, increased TAM abundance is associated with more aggressive disease.
View Article and Find Full Text PDFBackground: Normal epithelial cells and carcinoma cells can acquire invasiveness by epithelial-to-mesenchymal transition (EMT), a process of considerable cellular remodeling. The endosomal/lysosomal compartment is a principal site of intracellular protein degradation. Lysosomal cathepsin proteases are secreted during cancer progression.
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