Non-Contact Interaction Between Phorbol Myristate Acetate and Aqueous Alcohol Solutions Under Combined Magnetic Fields.

Previous research has demonstrated that a combined magnetic field (CMF) plays a critical role in modifying the properties of aqueous solutions, leading to an increase in the luminol-enhanced chemiluminescence of neutrophils. Using this model, the distant interaction between aqueous solutions was demonstrated, and the role of a CMF in the regulation of this phenomenon was established. In the current study, highly diluted (HD) phorbol myristate acetate (PMA) solution (the donor) was incubated with aqueous ethanol (the acceptor), both in a CMF-generating device and under geomagnetic field (GMF), for 0, 20, and 60 min.

Effect of Humid Air Exposed to IR Radiation on Enzyme Activity.

Water vapor absorbs well in the infra-red region of the electromagnetic spectrum. Absorption of radiant energy by water or water droplets leads to formation of exclusion zone water that possesses peculiar physico-chemical properties. In the course of this study, normally functioning and damaged alkaline phosphatase, horseradish peroxidase and catalase were treated with humid air irradiated with infrared light with a wavelength in the range of 1270 nm and referred to as coherent humidity (CoHu).

Stabilizing effects of hydrated fullerenes C₆₀ in a wide range of concentrations on luciferase, alkaline phosphatase, and peroxidase in vitro.

Hydrated fullerene (HyFnC60) is a highly hydrophilic supra-molecular complex consisting of unmodified С60 fullerene molecule enclosed into a hydrated shell. It has been shown in numerous experiments that aqueous solutions of HyFnC60 manifest a wide range of biological activities both in vivo and in vitro even at very low concentrations of HyFnC60. We used a spectrophotometric method and a method of biochemoluminescence to demonstrate that HyFnC60 in concentrations below 10(-9) M down to 10(-23) M stabilizes peroxidase, alkaline phosphatase, and bacterial luciferase against inactivation due to long-term incubation of the enzymes at room temperature and also against heat inactivation.

Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen and effects of C₆₀ fullerenes on blood chemiluminescence.

Background: Chronic obstructive pulmonary disease (COPD) is an inflammatory disease associated with reactive oxygen species (ROS) production. The aim of this study was to evaluate the effect of Hypoxen treatment and the effect of HyFnC₆₀ on ROS production in patients' blood.

Material/methods: ROS production in blood was estimated using chemiluminescence (CL) measurement with CL-amplifiers: luminol (LM), LM + zymosan (ZM) or lucigenin (LC) in the presence or absence of hydrated fullerenes (HyFnC₆₀) added to blood in low concentrations.

Reactive oxygen species, water, photons and life.

Unique properties of oxygen and of the reactions with reactive oxygen species (ROS) participation are considered, the multiple ways of ROS generation and utilization are discussed in view of evidence for the absolute necessity of ROS for the normal vital activity. Many difficulties in the realization of the real role of ROS in vital activity are caused by the attitude to them only as to chemical substances, while they should be considered in the first place as the major participants of continuous flows of highly non-linear processes in which electron excited species emerge. These processes play a significant role in energy and informational flows in all the living systems.

Water: a medium where dissipative structures are produced by a coherent dynamics.

The Belousov-Zhabotinsky phenomenon is analyzed in a framework where the dynamics of dissipative structures outlined by Prigogine is implemented through the collective dynamics produced in liquid water by Quantum Electrodynamics, which has received recently some experimental support. A mechanism allowing the appearance of self-produced oscillations is suggested.

The possible role of active oxygen in the memory of water.

Phenomena of long-term 'memory of water' imply that aqueous systems possessing it remain for a long period after the initial perturbation in an out-of equilibrium state without a constant supply of energy from the environment. It is argued here that various initial perturbations initiate development of a set of chain reactions of active oxygen species in water. Energy, in particular high grade energy of electronic excitation, released in such reactions can support non-equilibrium state of an aqueous system.

Reactive oxygen species (ROS): pathogens or sources of vital energy? Part 2. Bioenergetic and bioinformational functions of ROS.

In Part 1 of the review of experimental data concerning reactive oxygen species (ROS) production and use, their indispensable role in regulation of multiple physiologic processes is examined. Part 2 considers the unique property of reactions in which ROS participates, the generation of energy of electronic excitation (EEE). Because of incessant ROS cycling, EEE is continuously produced in aerobic organisms.

Reactive oxygen species--(ROS) pathogens or sources of vital energy? Part 1. ROS in normal and pathologic physiology of living systems.

Free radicals and reactive oxygen species (ROS) are considered to be dangerous pathogens as they may damage key molecular constituents of cells. However this concept approach does not take into account vital functions of ROS in normal physiology. Information has emerged that a substantial share of oxygen consumed by aerobic organisms is used for ROS production and that ROS are indispensable for regulation of multiple functions of living cells.

Influence of helio-geophysical factors (HGF) on environments, on health state and reliability of man's professional functioning, on processes of society development.

The present work reveals the helio-geophysical factors HGF) influence on natural surroundings, human and society, health state and professional reliability of human functioning in technogenic and social systems of extreme risk. The interdisciplinary complex investigations were performed on the social-population, organismal, organ cell and molecular levels. The HGF synergistic influence on a human state and functioning and the society especially within megalopolises' non-equilibrium geo-dynamic zones was studied in details.