Publications by authors named "Vladimir Plyuta"

Article Synopsis
  • The study highlights the diverse biological effects of β-ionone, a volatile organic compound, on bacterial cells, specifically focusing on Escherichia coli and Bacillus subtilis.
  • It was found that β-ionone induces oxidative stress in E. coli through the OxyR/OxyS regulatory system, but not through the SoxR/SoxS system, suggesting a specific response to the compound.
  • Additionally, at high concentrations, β-ionone can cause protein and DNA damage, while showing no oxidative stress effects in Bacillus subtilis, indicating the varying impact of β-ionone across different bacterial species.
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Volatile compounds emitted by bacteria can play a significant role in interacting with microorganisms, plants, and other organisms. In this work, we studied the effect of total gaseous mixtures of organic as well as inorganic volatile compounds (VCs) and individual pure volatile organic compounds (VOCs: ketones 2-nonanone, 2-heptanone, 2-undecanone, a sulfur-containing compound dimethyl disulfide) synthesized by the rhizosphere Pseudomonas chlororaphis 449 and Serratia plymuthica IC1270 strains, the soil-borne strain P. fluorescens B-4117, and the spoiled meat isolate S.

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This paper reports the results of the large-scale field testing of composite materials with antibacterial properties in a tropical climate. The composite materials, based on a cotton fabric with a coating of metal oxide nanoparticles (TiO2 and/or ZnO), were produced using high-power ultrasonic treatment. The antibacterial properties of the materials were studied in laboratory tests on solid and liquid nutrient media using bacteria of different taxonomic groups (Escherichia coli, Chromobacterium violaceum, Pseudomonas chlororaphis).

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Volatile organic compounds (VOCs) emitted by bacteria play an important role in the interaction between microorganisms and other organisms. They can inhibit the growth of phytopathogenic microorganisms, modulate plant growth, and serve as infochemicals. Here, we investigated the effects of ketones, alcohols, and terpenes on the colony biofilms of plant pathogenic strains and swimming motility, which can play an important role in the formation of biofilms.

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Bacteria and fungi emit a huge variety of volatile organic compounds (VOCs) that can provide a valuable arsenal for practical use. However, the biological activities and functions of the VOCs are poorly understood. This work aimed to study the action of individual VOCs on the bacteria , plants, and fruit flies .

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Article Synopsis
  • The study investigates the effects of two volatile organic compounds (VOCs), (-)-limonene and (+)-α-pinene, on bacterial cells, focusing on their mechanisms of action.
  • (-)-Limonene triggers multiple stress responses in bacteria by generating reactive oxygen species, leading to damage in DNA, proteins, and cell membranes, especially at high concentrations.
  • In contrast, (+)-α-pinene has a milder effect, primarily inducing a heat shock response, and both compounds interfere with bacterial protein refolding processes, with (-)-limonene being more effective at inhibiting this refolding.
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Microbial volatile organic compounds (VOCs) are cell metabolites that affect many physiological functions of prokaryotic and eukaryotic organisms. Earlier we have demonstrated the inhibitory effects of soil bacteria volatiles, including ketones, on cyanobacteria. Cyanobacteria are very sensitive to ketone action.

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In this study, we investigated the quorum sensing (QS) regulatory system of the psychrotrophic strain 94 isolated from spoiled refrigerated meat. The strain produced several -acyl--homoserine-lactone (AHL) QS signal molecules, with -(3-oxo-hexanoyl)--homoserine lactone and -(3-hydroxy-hexanoyl)--homoserine lactone as two main types. The and genes encoding an AHL synthase and a receptor regulatory protein, respectively, were cloned and sequenced.

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Many bacteria, fungi, and plants produce volatile organic compounds (VOCs) emitted to the environment. Bacterial VOCs play an important role in interactions between microorganisms and in bacterial-plant interactions. Here, we show that such VOCs as ketones 2-heptanone, 2-nonanone, and 2-undecanone inhibit the DnaKJE-ClpB bichaperone dependent refolding of heat-inactivated bacterial luciferases.

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Article Synopsis
  • The study focuses on how volatile organic compounds (VOCs) from certain bacteria can impact biofilm formation in Agrobacterium tumefaciens, which causes crown-gall disease in plants.
  • Through dual culture assays, it was found that the VOCs from Pseudomonas and Serratia strains not only suppressed biofilm formation but also killed established A. tumefaciens cells.
  • Specific compounds, like certain ketones and dimethyl disulfide (DMDS), show potential as natural protectants for plants against these harmful bacteria due to their ability to inhibit biofilm-related virulence.
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In the natural environment, bacteria predominantly exist in matrix-enclosed multicellular communities associated with various surfaces, referred to as biofilms. Bacteria in biofilms are extremely resistant to antibacterial agents thus causing serious problems for antimicrobial therapy. In this study, we showed that different plant phenolic compounds, at concentrations that did not or weakly suppressed bacterial growth, increased the capacity of Pseudomonas aeruginosa PAO1 to form biofilms.

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