Immunocorrection Therapy: Drugs and Prospects.

The analysis has been made for the basic immunocorrection drugs, used in clinical practice. By their features, the drugs are divided into 6 groups: immunoactive structures of pathogens, polyelectrolites, inductors of IFN-alpha, thymic hormones and their analogs, bone marrow regulators (myelopeptides) and cytokines. The first 3 groups of drugs are characterized by the inductive mechanism of action, whereas thymic hormones, myelopeptides and cytokines by the substitutive mechanism.

Methods for preparation of recombinant cytokine proteins V. mutant analogues of human interferon-gamma with higher stability and activity.

Mutant analogues of recombinant human immune interferon (IFN-gamma) with higher stability and biological activity were prepared. Depending on the analogue, protein structure modification might involve introduction of an intramonomer disulfide bond (through replacements of Glu7Cys and Ser69Cys), C-terminal shortening by 10 amino acid residues, as well as Gln133Leu substitution in truncated variant. Isolation, purification, and renaturation of the IFN-gamma analogues expressed in Escherichia coli as inclusion bodies were performed according to the scheme developed earlier for wild-type protein.