Comparative biochemical characterization of 5'-phosphodiesterase and phosphomonoesterase from barley malt sprouts.

A comparative biochemical characterization is described of two competing enzymes in the production of flavoring 5'-ribonucleotides, barley malt sprouts 5'-phosphodiesterase (5'-PDE) and phosphomonoesterase (PME). Fractionation of these two enzymes and partial purification of 5'-PDE were achieved by a combination of thermal treatments and precipitation with acetone. With synthetic substrates, under standard assay conditions, 5'-PDE and PME had maximum activities at pH 8.

Isolation and characterization of cerebrosides of the hydrocarbon-assimilating yeast Yarrowia lipolytica.

Yarrowia lipolytica yeast was grown batchwise on n-hexadecane as the carbon and energy source. Two cerebroside species were quantitatively isolated from sphingolipid fractions of total lipids by a combination of column chromatography and preparative high-performance thin-layer chromatography. The cerebroside content accounted for 1.

Dependence of the sphingoid bases concentration on growth phase and temperature in the yeast Yarrowia lipolytica.

The aim of this research was to investigate the effect of growth phase and temperature on the concentrations of the individual molecular species of sphingoid based obtained by acid hydrolysis of total sphingolipids from the yeast Yarrowia lipolytica. Our results showed C18 phytosphingosine to be the major long-chain base in Y. lipolytica regardless of growth phase or temperature.