The present work aims to investigate the kinetic characteristics of homodimer enzyme L-lysine α-oxidase from cf. Rifai VKM F-4268D, taking into account allosteric effects. The enzyme was first shown to reveal positive cooperativeness, =2.
View Article and Find Full Text PDFThis work studied the effect of gossypol on the mitochondrial respiratory chain of Yarrowia lipolytica. The compound was shown to inhibit mitochondrial electron transfer and stimulate generation of reactive oxygen species. The inhibition kinetics in oxidation of various substrates (NADH, succinate, α-glycerophosphate and pyruvate + malate) by isolated mitochondria was investigated.
View Article and Find Full Text PDFEquations for calculation of constants of enzyme activation and nontrivial types of enzyme inhibition, which are not reported in the literature, have been deduced. Examples of using these equations are also presented.
View Article and Find Full Text PDFEquations for calculation of the initial rates of activated enzymatic reactions and the inhibited enzymatic reactions, unavailable in experimental enzymology, were obtained. Examples of practically using of these equations are given.
View Article and Find Full Text PDFA parametrical classification of types of enzymatic reactions that counts 15 types-7 inhibited reactions, 7 activated ones, and 1 type of initial (uninhibited and nonactivated) reaction-is considered. The system permits taking into account the number of parameters of enzymatic reactions and symmetricity of a course of their change.
View Article and Find Full Text PDFThe possibility of construction of the three-dimensional (unfolded and folded) K'm V'I rectangular coordinate systems convenient for vector representation of inhibited and activated enzymatic reactions as well as of a two-dimensional K'm V' scalar rectangular coordinate system convenient for diagrammatic representation of enzymatic reactions is considered. The perspectives of using the properties of the three-dimensional L vectors and their scalar L projections for data analysis of enzyme inhibition and activation are analyzed.
View Article and Find Full Text PDF