Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications.

The extracellular enzyme with oxidase function was extracted from the Neonothopanus nambi luminescent fungus by using mild processing of mycelium with β-glucosidase and then isolated by gel-filtration chromatography. The extracted enzyme is found to be a FAD-containing protein, catalyzing phenol co-oxidation with 4-aminoantipyrine without addition of HO, which distinguishes it from peroxidases. This fact allowed us to assume that this enzyme may be a mixed-function oxidase.

Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system.

Background: Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield.

Two forms of substrate for the bioluminescent reaction in three species of basidiomycetes.

The luminescent response of the enzymatic system of on the cold and hot extracts from cell-free culture liquids of sp. and was examined. The greatest influence on the light emission produced by the luminescent system of was provided by the temperature at which the probes were prepared for assay.

Detonation Nanodiamonds as a New Tool for Phenol Detection in Aqueous Medium.

This paper demonstrates the effectiveness of using detonation nanodiamonds (DNDs) for detecting phenol in aqueous medium. The study has shown that the catalytic effect of DNDs in the oxidative azo coupling reaction (phenol-4-aminoantipyrine-hydrogen peroxide) is produced by trace amounts of iron and copper ions adsorbed on the surface of nanoparticles. The effectiveness of DNDs as a catalyst is determined by the amounts of these adsorbates and can be enhanced by a factor of two by additional adsorption of these ions onto the nanoparticles.

The Formation of Crystal Defects in a Fe-Mn-Si Alloy Under Cyclic Martensitic Transformations.

Formation of crystalline defects due to cyclic martensitic transformations (CMT) in the iron-manganese Fe-18 wt.% Mn-2 wt.% Si alloy was investigated using X-ray diffractometry.

A Randomized Phase II/III Study of Naptumomab Estafenatox + IFNα versus IFNα in Renal Cell Carcinoma: Final Analysis with Baseline Biomarker Subgroup and Trend Analysis.

Purpose: To prospectively determine the efficacy of naptumomab estafenatox (Nap) + IFNα versus IFN in metastatic renal cell carcinoma (RCC).

Experimental Design: In a randomized, open-label, multicenter, phase II/III study, 513 patients with RCC received Nap (15 μg/kg i. v.

Biodistribution of Different Sized Nanodiamonds in Mice.

The particle size is one of critical parameters influencing the biodistribution of detonation nanodiamonds (DND) after their administration into the body. As DNDs are prone to aggregation, the difference between their sizes in aqueous and physiological solutions has to be taken into account. Radioactive I125-BSA molecules were covalently immobilized on DNDs divided in three fractions of different average size.

The Chemical Basis of Fungal Bioluminescence.

Many species of fungi naturally produce light, a phenomenon known as bioluminescence, however, the fungal substrates used in the chemical reactions that produce light have not been reported. We identified the fungal compound luciferin 3-hydroxyhispidin, which is biosynthesized by oxidation of the precursor hispidin, a known fungal and plant secondary metabolite. The fungal luciferin does not share structural similarity with the other eight known luciferins.

Near-IR nonlinear optical filter for optical communication window.

We report on a high performance nonlinear optical filter for the telecommunication window that employs detonation nanodiamonds (NDs). The nanosecond Z-scan experiments revealed that the heavy water ND suspensions enable strong optical limiting in the wavelength range of 1400-1675 nm. We observed an enhancement of the optical limiting performance in the blue part of the communication window.

Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation.

The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi.

Size effect on the optical limiting in suspensions of detonation nanodiamond clusters.

We report on the optical limiting (OL) in stable aqueous suspensions of detonation nanodiamond (ND) clusters with average size of 50, 110, and 320 nm. The nanosecond Z-scan measurements at wavelength of 532 nm revealed that the larger the cluster size, the better the OL performance and the higher the ray stability of the ND suspension. Our analysis showed that the nonlinear scattering and the nonlinear absorption are the dominant mechanisms of OL in aqueous ND suspensions.