Publications by authors named "Vladimir A Nemtsov"
Article Synopsis
- Developing effective oligonucleotides for RNA interference (RNAi) is complex due to variable results and the need for significant computational support in building genome-wide RNAi libraries.
- The authors introduce a quick and practical algorithm for designing short hairpin RNAs (shRNAs) by analyzing thousands of RNAi experiments, identifying key parameters, specifically terminal duplex asymmetry and duplex stability, that correlate with higher silencing efficiency.
- Testing their predictions, the researchers designed 83 shRNAs demonstrating that slight adjustments in duplex stability can significantly impact silencing efficiency, achieving results comparable to the best existing prediction methods in RNAi design.
Background: Computer programs for the generation of multiple sequence alignments such as "Clustal W" allow detection of regions that are most conserved among many sequence variants. However, even for regions that are equally conserved, their potential utility as hybridization targets varies. Mismatches in sequence variants are more disruptive in some duplexes than in others.
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