[Problems and opportunities to improve diagnosis of asthma and chronic obstructive pulmonary disease in Russia: resolution of advisory board].

Asthma and chronic obstructive pulmonary disease remain major problems of medicine, and still there is need to improve the level and quality of diagnosis of these diseases. Primary care physicians (general practitioners, therapists) should be involved widely and actively in this process. To simplify the diagnosis, special questionnaires have been developed, they can be used in a real clinical practice.

The impact of demographic disparities in the presentation of sarcoidosis: A multicenter prospective study.

Objective: To study how demographic differences impact disease manifestation of sarcoidosis using the WASOG tool in a large multicentric study.

Methods: Clinical data regarding 1445 patients with sarcoidosis from 14 clinical sites in 10 countries were prospectively reviewed from Feb 1, 2020 to Sep 30, 2020. Organ involvement was evaluated for the whole group and for subgroups differentiated by sex, race, and age.

Management of Cough in Patients with Chronic Obstructive Pulmonary Disease: Results of the Multicenter Randomized Placebo-Controlled Clinical Trial.

Background: Chronic cough declines quality of life and increases risk of complications in patients with chronic obstructive pulmonary disease (COPD). Reducing cough severity and associated negative effects is important therapeutic goal in COPD. Rengalin with anti- and protussive activity is based on technologically processed antibodies to bradykinin, histamine and morphine.

[The concept of chronic obstructive pulmonary disease clinical control as a decision - making tool in real clinical practice for optimizing of basic pharmacotherapy].

The main goals of COPD therapy are to achieve clinical stability with minimal clinical manifestations and low risk of relapse. The proposed COPD control concept by analogy with asthma has not been quite well characterized yet. COPD control is defined as "the long - term maintenance of a clinical situation with a low impact of symptoms on the patient's life and absence of exacerbations.

Scope and new horizons for implementation of m-Health/e-Health services in pulmonology in 2019.

The reason for this review based on the results of many meta- analyses is the great assessed difference in the methods of most studies in e-Health, telemedicine and tele-rehabilitation. It consists of different understanding of new terms, using different hard- and software, including criteria, different methodology of patient's treatment and its evaluation. This status suggests that first of all m-Health/e-Health requires a unique ontology of terms using and methodology of studies comparing.

ADEPTUS: a discovery tool for disease prediction, enrichment and network analysis based on profiles from many diseases.

Motivation: Large-scale publicly available genomic data on many disease phenotypes could improve our understanding of the molecular basis of disease. Tools that undertake this challenge by jointly analyzing multiple phenotypes are needed.

Results: ADEPTUS is a web-tool that enables various functional genomics analyses based on a high-quality curated database spanning >38, 000 gene expression profiles and >100 diseases.

[Dynamics of clinical, X-ray and functional data during treatment of patients with sarcoidosis].

Aim: To analyze the literature data and the results of original studies on the respiratory function in patients with sarcoidosis.

Material And Methods: The study included 1199 patients with sarcoidosis (67.8% women and 32.

[The use of methotrexate for the treatment of sarcoidosis (data from a controlled prospective study)].

We estimated the effectiveness and safety of methotrexate monotherapy of sarcoidosis in 63 patients refractory to the previous treatment. Methotrexate was given weekly at a dose of 5-20 mg. 54% of the patients reported improvement of their condition.

[X-ray, laboratory, and functional parallels in intrathoracic sarcoidosis].

Aim: to compare respiratory function and laboratory data with the radiographic stages of intrathoracic sarcoidosis.

Subjects And Methods: Three hundred and eleven patients (70.4% for women and 29.

Tuberculosis in Russia. Its history and its status today.

The history of the Russian tuberculosis (TB) program, dating from the second part of the 19th century until today, is presented, and its achievements and failures are analyzed. The epidemiology of TB is described, and the effects of the TB program initiatives are described and analyzed. Multidrug-resistant TB and HIV coinfection represent severe threats to TB control effectiveness in Russia.

[Multicenter retrospective analysis of the patients with sarcoidosis with a 10 year interval in observations].

Comparison of the state of 83 patients with histologically confirmed sarcoidosis observed with a 10 year interval revealed remission in 47% of the cases. The main factors having negative effect on prognosis of the disease included extrapulmonary symptoms, the use ofcorticosteroids (at all stages, especially at stage I and in Lofgren syndrome) and antituberculosis drugs, positive TB test. Risk factors of relapses were stage II sarcoidosis, the use of systemic corticosteroids in patients with Lofgren syndrome and antituberculosis drugs, initially low FEV1/FVLC ratio and the number of lymphocytes in peripheral blood.

[Comparative analysis of the treatment of inpatients with community-acquired pneumonia and its different outcomes].

The aim of the work was to compare the quality of medical care provided to patients with severe community-acquired pneumonia (CAP) and its different outcomes. (complete recovery, or death--25 patients in either group). In the latter group, the patients did not undergo adequate clinical and instrumental examination at the pre-hospital stage).

Automated sample preparation for CE-SDS.

Traditionally, CE with SDS (CE-SDS) places many restrictions on sample composition. Requirements include low salt content, known initial sample concentration, and a narrow window of final sample concentration. As these restrictions require buffer exchange for many sample types, sample preparation is often tedious and yields poor sample recoveries.

Automated tryptic digestion procedure for HPLC/MS/MS peptide mapping of immunoglobulin gamma antibodies in pharmaceutics.

The rapid growth of antibody drugs and drug candidates in the biopharmaceutical industry has created a demand for automated proteolytic digestion to assist in pharmaceutical stability studies, identity assays and quality control of these therapeutic proteins. Here, we describe the development of a fully automated proteolytic digestion procedure for monoclonal antibodies in solution, which requires a high concentration of denaturants for unfolding. The antibody samples were placed in a 96-well plate or in 0.

Investigation of N-terminal glutamate cyclization of recombinant monoclonal antibody in formulation development.

The N-terminal glutamic acid (Glu) can be cyclized to form pyroglutamate (pGlu). Recent studies have suggested that N-terminal pGlu formation is an important posttranslational or co-translational event and is greatly facilitated by the enzyme glutaminyl cyclase, although the impact of the N-terminal cyclization on the potency and overall stability of mAbs is not been well known. Since most recombinant monoclonal antibodies (mAbs) contain glutamic acid and/or glutamine at their N-terminus, understanding the cyclization mechanisms may shed light on the factors that control the pGlu formation in therapeutic mAb development.

[Evaluation of the efficiency of various treatment regimens for patients with newly-detected sarcoidosis in the Republic of Tatarstan].

The natural history of sarcoidosis was analyzed in 279 patients with its history of as long as 2 years among the 832 with this disease registered in the Republic of Tatarstan in 1999 to 2002. The patients were divided into 3 groups: (1) 79 (28.3%) receiving systemic glucocorticosteroids (SGS); (2) 146 (52.

Formation of pyroglutamic acid from N-terminal glutamic acid in immunoglobulin gamma antibodies.

The status of the N-terminus of proteins is important for amino acid sequencing by Edman degradation, protein identification by shotgun and top-down techniques, and to uncover biological functions, which may be associated with modifications. In this study, we investigated the pyroglutamic acid formation from N-terminal glutamic acid residues in recombinant monoclonal antibodies. Almost half the antibodies reported in the literature contain a glutamic acid residue at the N-terminus of the light or the heavy chain.

[Comparison of acute bronchodilator tests in patients with chronic obstructive lung disease and bronchial asthma].

Three hundred and thirty-five patients with bronchial asthma (BA) and 534 patients with chronic obstructive lung disease (COLD) were examined. Before and after tests using salbutamol, phenoterol, ipratropium bromide and a fixed combination of phenoterol and ipratropium bromide, the forced expiratory flow-volume curve was recorded in the patients on each visit (1008 and 1398 visits of patients with BA and COLD, respectively). The study was controlled open-labeled, and prospective.

Fluoroquinolones for treating tuberculosis.

Background: Fluoroquinolones are sometimes used to treat multiple-drug-resistant and drug-sensitive tuberculosis. The effects of fluoroquinolones in tuberculosis regimens need to be assessed.

Objectives: To assess fluoroquinolones as additional or substitute components to antituberculous drug regimens for drug-sensitive and drug-resistant tuberculosis.

Mechanisms of the effect of 1,1-dimethyl-3-hydroxybutyl phosphonic acid derivatives on synaptic transmission in neuromuscular junction.

We studied the effect of homologues derivatives of 1,1-dimethyl-3-hydroxybutyl phosphonic acid on synaptic transmission in frog neuromuscular junction. Here we reviewed general mechanisms of inhibition of the postsynaptic current.

[Sarcoidosis and pregnancy: data from scientific publications and a clinical case].

The paper analyzes the literature on sarcoidosis and pregnancy, available from the MedLine bases and Russian journals. It also presents a clinical case of sarcoidosis developed and recurred in a female after the first and repeated pregnancy. It is concluded that pregnancy in sarcoidosis may be preserved in most cases.

[A comparative study of lymphocytic apoptosis and ventilation disorders in patients with pulmonary tuberculosis].

Due to the increasing interest in studies of apoptosis (programmed cell death) in the pathogenesis of many diseases and to the emergence of data on the direct impact of apoptotic disorders on the course of bronchial asthma and the magnitude of the obstructive syndrome, this study was undertaken to investigate apoptosis in patients with new-onset pulmonary tuberculosis who had varying bronchial obstructive syndrome. Twenty-eight patients with new-onset tuberculosis, including 25 (89.3%), 2(7.

[Specific features of cardiorespiratory system in patients with intrathoracic sarcoidosis].

A hundred thirty eight patients with pulmonary sarcoidosis were examined for the status of the respiratory and cardiovascular systems. There is a trend for external respiratory parameters to decrease as the X-ray stage increases (by 30-50% in Stage IV). The revealed cardiovascular changes in patients with different X-ray stages did not differ significantly and there was no clear correlation with the activity of a process and external respiratory parameters.