Both ADP and thrombin regulate arteriolar thrombus stabilization and embolization, but are not involved in initial hemostasis as induced by micropuncture.

Objective: Thrombosis and embolization are main causes of morbidity and mortality. Up to now, the relative importance of mediators involved is only partly known. It was the aim of this study to investigate the involvement of ADP and thrombin in subsequent phases of arteriolar hemostasis and thromboembolism in vivo.

In vivo visualization of vascular leakage in photochemically induced cortical infarction.

The photothrombotic model for stroke was originally described as a focal cortical infarction resulting from occlusive thrombosis. However, subsequent studies have shown evidence for extravasation of water and proteins using ex vivo techniques. The aim of the present study was to determine the role of vascular leakage in the pathophysiology of photochemically induced infarction in vivo.

Real-time detection of activation patterns in individual platelets during thromboembolism in vivo: differences between thrombus growth and embolus formation.

Knowledge on single platelet behavior and intracellular mechanisms during thromboembolism in vivo is scarce. In the present study, we used a new method that enables real-time detection and quantification of activation of individual platelets participating in a thromboembolic process in vivo, using their intracellular free Ca(2+) concentration ([Ca(2+)](i)) as a marker of activation. Isolated platelets were labeled with the Ca(2+)-sensitive fluorescent probe fluo-3 and injected into anesthetized rabbits so that 0.