Substitution of the Axial Type 1 Cu Ligand Affords Binding of a Water Molecule in Axial Position Affecting Kinetics, Spectral, and Structural Properties of the Small Laccase Ssl1.

Multicopper oxidases use Cu ions as cofactors to oxidize various substrates. High reduction potential at Type 1 Cu is considered as crucial for effective catalysis. Previous studies have shown that replacing the axial methionine ligand of the Type 1 Cu with leucine or phenylalanine leads to an increased reduction potential, but not always to higher enzyme activity.

Discovery of a new class of bacterial heme-containing CC cleaving oxygenases.

Previously, some bacteria were shown to harbour enzymes capable of catalysing the oxidative cleavage of the double bond of t-anethole and related compounds. The cofactor dependence of these enzymes remained enigmatic due to a lack of biochemical information. We report on catalytic and structural details of a representative of this group of oxidative enzymes: t-anethole oxygenase from Stenotrophomonas maltophilia (TAO).

Cell-free enzyme cascades - application and transition from development to industrial implementation.

In the vision to realize a circular economy aiming for net carbon neutrality or even negativity, cell-free bioconversion of sustainable and renewable resources emerged as a promising strategy. The potential of in vitro systems is enormous, delivering technological, ecological, and ethical added values. Innovative concepts arose in cell-free enzymatic conversions to reduce process waste production and preserve fossil resources, as well as to redirect and assimilate released industrial pollutions back into the production cycle again.

Integrating Carbohydrate and C1 Utilization for Chemicals Production.

In the face of increasing mobility and energy demand, as well as the mitigation of climate change, the development of sustainable and environmentally friendly alternatives to fossil fuels will be one of the most important tasks facing humankind in the coming years. In order to initiate the transition from a petroleum-based economy to a new, greener future, biofuels and synthetic fuels have great potential as they can be adapted to already common processes. Thereby, especially synthetic fuels from CO and renewable energies are seen as the next big step for a sustainable and ecological life.