In vitro evaluation of bilayer membranes of PLGA/hydroxyapatite/β-tricalcium phosphate for guided bone regeneration.

Membranes for guided bone regeneration represent valuable resources, preventing fibroblast infiltration and aiding anatomical bone reconstruction. Nonetheless, available membranes lack bone regenerative capacity, suitable mechanical behavior, or adequate degradation profile. Therefore, to overcome these limitations, this study developed bilayer membranes with a dense layer (dry phase inversion) of PLGA (poly(lactic-co-glycolic acid)):HAp (hydroxyapatite) - 95:05 (wt%) - and an electrospun layer of PLGA and HAp:β-TCP (β-tricalcium phosphate) with ratios of 60:40, 70:30 and 85:15 (wt%), evaluating its mechanical, morphological and in vitro properties.