Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis.

The advent of mass cytometry (CyTOF) has permitted simultaneous detection of more than 40 antibody parameters at the single-cell level, although a limited number of metal-labeled antibodies are commercially available. Here we present optimized and scalable protocols for conjugation of lanthanide as well as bismuth ions to immunoglobulin (Ig) using a maleimide-functionalized chelating polymer and for characterization of the conjugate. The maleimide functional group is reactive with cysteine sulfhydryl groups generated through partial reduction of the Ig Fc region.

Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis.

Background: Basophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies.

Objective: We attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample.

[Treatment of anorexia nervosa].

Treatment of anorexia nervosa integrates psychiatric and medical aspects. Elements of analytically oriented (not true psychoanalysis) and behavioral forms of psychotherapy are utilized. Emphasis should be placed in the setting, patient-therapist relation, and alliance with the family.

[Cephalometric study of the intermaxillary sagittal relationship and final clinical diagnosis].

Due to the difficulties in finding a reliable cephalometric method to determine the true intermaxillary sagital relationship, the usefulness of the Freeman (AXB) and the Beatty (AXD) cephalometric methods are studied and, furthermore, which of them best demonstrate said sagital relationship. To perform this a comparative analysis of cephalometric methods AXB and AXD with the clinical cephalometric diagnosis were carried out using a sample of 33 individuals of both genders, between the ages 18 and 26 years, without orthodontic treatment, complete dentition and not taking into consideration third molars. The frequency distributions of class I, II and III were obtained through values given by methods AXD and AXB.