Publications by authors named "Vitvitskiĭ V"

We studied the influence of various gamma-ray doses on the death pattern of Wistar rats and the changes in lethality after introduction of RNA isolated from various tissues in the organism. All studied RNA introduced into the irradiated animals proved to clearly stimulate repair of the cells damaged by radiation and to decrease both cytotoxic and cytogenetic effects of gamma-rays. The most pronounced effect on the cells was specific for RNA isolated from the matching tissue.

View Article and Find Full Text PDF

Aim: To study pharmacokinetics of liposomal daunorubicine DaunoXome and daunorubicine (rubomycin) associated with red cells: to assess their effectiveness and toxicity in patients with acute leukemia.

Materials And Methods: 7 patients with resistant or recurrent acute leukemia entered the trial. Of them 2 patients had acute myeloid leukemia.

View Article and Find Full Text PDF

In isotonic medium, daunorubicin in the concentration range of 0.5-5.0 mg/ml of cells and doxorubicin in the concentration range of 0.

View Article and Find Full Text PDF

The effect of K2Cr2O7 and (CH3COO)2Pb on the mutagenic activity of gamma rays was studied by a micronucleus test in mouse bone marrow polychromatocytes. Acute and chronic combined actions of the two factors were investigated. Chromium ions (VI) enhanced mutagenic effects of gamma rays in both acute and chronic experiments.

View Article and Find Full Text PDF

Amphotericin B (1-3 mg/l) decreases the ATP content in erythrocytes by 11-26% and stimulates the K+ efflux but has no effect on the adenylate pool. Adenosine added to the erythrocyte suspension increases the adenylate pool, maintains a high intracellular ATP level for 6-8 hours of incubation at 37 degrees C and diminishes the amphotericin B-induced leakage of K+. Incubation of erythrocytes without glucose for 4-5 hours leads to a 20-50% loss of ATP accompanied by a significant reduction of the adenylate pool.

View Article and Find Full Text PDF

In the accompanying paper the process of erythrocyte passage through pores of membrane nuclear filters has been modelled. In order to test the model we have studied how the filtration rate of human erythrocyte suspension depends on osmoticity of the resuspending medium at various experimental conditions. We have tested and verified the model conclusion that the zero filtration rate is reached at the critical osmoticity value which depends only on geometrical parameters of the pore and the cell and not on other properties of the erythrocyte.

View Article and Find Full Text PDF

When incubated in the isotonic medium containing daunorubicin, human erythrocytes bound daunorubicin. At the initial concentration of daunorubicin in the medium of not higher than 1 mg/ml, the suspension hematocrit of about 30 per cent and the temperature of 25 degrees C the maximum binding of daunorubicin was achieved in approximately 30 minutes and was equal to about 80 per cent of the initial content. When the temperature was lowered from 30 to 15 degrees C the initial rate of the daunorubicin binding by the erythrocytes decreased several times.

View Article and Find Full Text PDF

A mathematical model is constructed, which quantitatively describes the rate of erythrocyte passage through pores of nuclear membrane filters during filtration of a diluted erythrocyte suspension upon action of a constant hydrostatic pressure. The following main factors have been taken into account: geometrical constraints linking the surface area of the erythrocyte membrane, the erythrocyte volume and the geometrical parameters of the filter pores; mechanical characteristics of the erythrocyte membrane; viscosity of the intracellular content. Analysis of the model allows us to conclude that it is possible to extract information about all above erythrocyte characteristics from the experimental curves describing, dependency of the filtration rate of the erythrocyte suspension from the osmoticity of the outer medium.

View Article and Find Full Text PDF

Efficacy of the combined therapy of experimental salmonellosis was studied on albino rats with using ampicillin, gentamicin, tetracycline and immunomodulators such as levamisole and quadevite. The influence of the combined chemoimmunotherapy on the indices of the nonspecific resistance of the animals i.e.

View Article and Find Full Text PDF

The study of two flow methods (constant flow method and initial flow rate method) has been conducted to specify and compare methodological approaches to quantitation of red cell deformability. Washed red cells were resuspended in saline medium with glucose and albumin and examined for deformability by the above methods under different conditions. The parameters are presented to characterize flow curves obtained with the constant flow method.

View Article and Find Full Text PDF

An attempt to correct with quadevit immunodepression due to prophylactic administration of benzylpenicillin and gentamicin for 6 days provided positive results. There was a significant increase in the quantities of Ig 19S and Ig 7S after the animal immunization with the bacterial antigen, an increase in the number of the AFCs in the spleen of the albino mice in response to administration of sheep erythrocytes and an increase in the survival rate of the animals with salmonellosis. The simultaneous use of quadevit with cefamezine and erythromycin did not lower the unfavourable influence of the antibiotics on the immune status of the animals.

View Article and Find Full Text PDF

Embryonal nervous tissue from Wistar rats was transplanted into male rats of Wistar and August strains. Activity of eight enzymes belonging to various systems was estimated in brain cortex of rats recipients within 36 days after the transplantation. Lactate dehydrogenase, alanine aminotransferase, acid phosphatase, 5'-nucleotidase, ATPase and aldolase exhibited the dissimilarly decreased rate of activity in brain cortex of Wistar rats after transplantation as compared with the enzymatic activity in intact animals of this strain, while activity of alkaline phosphatase and esterases hydrolyzing alpha-naphthyl acetate was increased.

View Article and Find Full Text PDF

Nervous tissue of 17-days old rat embryos was transplanted into lateral ventricle of the brain of adult rats. 15 days after transplantation esterase activity was analyzed from transplant tissue and flanking regions of cerebral cortex. Isoenzymes were shown to activate their activity after embryonic tissue transplantation either in transplants or flanking regions.

View Article and Find Full Text PDF

The use of ampicillin, benzylpenicillin, gentamicin, erythromycin, cefamezine or cephaloridine in combination with levamisole aimed at increasing their efficacy and correcting the adverse reactions was studied. Immunological activity of the antibiotics was comparatively characterized by using standard methods. It was shown that the character of the antibiotic effect on the basic factors of nonspecific resistance and immunity was heterogeneous.

View Article and Find Full Text PDF

The activity of lactate dehydrogenase (LDH), indophenol oxidase, aspartate aminotransferase (AsAT), alkaline phosphatase, acid phosphatase and aldolase at different stages of rat development was measured. We have also determined changes in the activity of these enzymes resulting from transplantation of embryonic nerve tissue (ENT) into the brain of adult animals. During development from the embryo to the adult animal, LDH and AsAT activities increased, while alkaline phosphatase activity diminished.

View Article and Find Full Text PDF

The DNA and RNA synthesis in the cells of the brain cortex of intact rats and animals subjected to hypoxia, hypoxia with subsequent transplantation or by the local brain injury has been investigated. The DNA synthesis changes insignificantly in the case of hypoxia, it enhances slightly in the area of the injury and increases much more after transplantation. The RNA synthesis decreases considerably immediately after hypoxia and decreases much more 120 days later.

View Article and Find Full Text PDF

Incorporation of 14C-adenine (imp/min per mg) into RNA of brain tissue and into fractions of neurones and glial cells of cerebral cortex was studied rats with diffuse damage (hypoxia), with local injury of brain tissue and after transplantation of embryonal nervous tissue. The diffuse damage decreased a number of cells in the fraction enriched with neurones and, therefore, diminished incorporation of the marker into the whole preparation. No significant changes was found in a number of glial cells.

View Article and Find Full Text PDF

The luciferase preparation obtained from fireflies Luciola mingrelica has entrapped into the human erythrocytes by means of reversible osmotic lysis. The addition of luciferin to such erythrocytes leads to the appearance of luminescence, conditioned by the entrance of luciferin into the cells. Luciferin is uniformly distributed between cells and external medium.

View Article and Find Full Text PDF

The changes in biochemical processes in brain cortex in rats with experimental hypoxia and hypoxia with consequent transplantation of embryonic nervous tissue into brain of adults have been studied. A small increase (2-3 times) in incorporation of biosynthesis precursors was observed as a result of transplantation both in normal and in hypoxic rats. These changes could be observed 100 days after the transplantation.

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_sessionuhqd5667oti62fr45asc0fdtn4s0ufcb): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once