Publications by authors named "Vito Flavio Licciulli"
Intestinal microorganisms impact health by maintaining gut homeostasis and shaping the host immunity, while gut dysbiosis associates with many conditions, including autism, a complex neurodevelopmental disorder with multifactorial aetiology. In autism, gut dysbiosis correlates with symptom severity and is characterised by a reduced bacterial variability and a diminished beneficial commensal relationship. Microbiota can influence the expression of host microRNAs that, in turn, regulate the growth of intestinal bacteria by means of bidirectional host-gut microbiota cross-talk.
View Article and Find Full Text PDFSummary: ITSoneWB (ITSone WorkBench) is a Galaxy-based bioinformatic environment where comprehensive and high-quality reference data are connected with established pipelines and new tools in an automated and easy-to-use service targeted at global taxonomic analysis of eukaryotic communities based on Internal Transcribed Spacer 1 variants high-throughput sequencing.
Availability And Implementation: ITSoneWB has been deployed on the INFN-Bari ReCaS cloud facility and is freely available on the web at http://itsonewb.cloud.
Article Synopsis
- Folding reporters are proteins that indicate certain traits, like antibiotic resistance, but their effectiveness can decrease when linked to poorly folding proteins; we developed a method to select well-folded proteins from any intronless genome using TEM-1 β-lactamase.* -
- By creating a "domainome" from these proteins, we ensure they are well expressed and soluble, which allows us to study their structure and function more easily.* -
- The protocols involve cloning the "domainome" in a phage display system and using advanced next-generation sequencing to analyze the selection process, providing detailed insights into the diversity and features of the protein fragments collected.*
Summary: DNAfan (DNA Feature ANalyzer) is a tool combining sequence-filtering and pattern searching. DNAfan automatically extracts user-defined sets of sequence fragments from large sequence sets. Fragments are defined by annotated gene feature keys and co- or non-occurring patterns within the feature or close to it.
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