Publications by authors named "Vitika Chauhan"
Article Synopsis
- - The study investigates the role of miRNAs in the seminal plasma extracellular vesicles (SP-EVs) from high-fertile (HF) and low-fertile (LF) Sahiwal bulls, focusing on how these molecules might affect fertility through genetic and molecular signaling.
- - SP-EVs were isolated and analyzed, showing different sizes and particle concentrations between HF and LF bulls, but the overall differences in size and concentration were not statistically significant.
- - High-throughput miRNA sequencing identified 310 miRNAs, with significant differences in expression levels between HF and LF bulls, particularly highlighting bta-miR-195, which was found to be highly expressed in HF spermatozoa and may be linked to improved fertility
Backgroud: Before fertilization, spermatozoa undergo a crucial maturation step called capacitation, which is a unique event regulates the sperm's ability for successful fertilization. The capacitation process takes place as the spermatozoa pass through the female reproductive tract (FRT). Dihydrolipoamide dehydrogenase (DLD) protein is a post-pyruvate metabolic enzyme, exhibiting reactive oxygen species (ROS) production which causes capacitation.
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- Buffalo bulls play a crucial role in the Indian dairy industry, with the fertility quality of semen donating bulls directly impacting dairy farm efficiency.
- The study analyzed extracellular vesicles (EVs) in seminal plasma, comparing size, concentration, and protein profiles from high-fertility (HF) and low-fertility (LF) bulls, but found no significant size or concentration differences.
- It identified 1,862 proteins in HF bulls and 1,807 in LF bulls, with 87 proteins more abundant in the HF group, many linked to critical reproductive processes such as sperm motility and fusion with oocytes.