Publications by authors named "Vitaly N Maslenny"
Article Synopsis
- Previous studies show that using activated charcoal can partially restore albumin's function in uraemic plasma, especially at pH 3, although this leads to loss of vital plasma components.
- The research tested albumin's melting curves and binding abilities after extraction at different pH levels (7.2, 5.08, and 3.0), finding that pH 5.08 fully restored its ability to bind key ligands.
- The study suggests that activated carbon with proper pH adjustment can effectively remove toxic substances from the plasma, and the melting curve of albumin serves as a new method for assessing its condition and the effectiveness of detoxification processes.
Albumin, bilirubin, and activated carbon: new edges of an old triangle.
Artif Cells Blood Substit Immobil Biotechnol
March 2002
The problem of interaction of human serum albumin (HSA), unconjugated bilirubin (UB) and high porosity activated HSGD carbons is investigated in this study. The decrease of UB to HSA molecular ratio by more than 300 times was demonstrated while the batch experiments in HSA-UB admixtures after contact with HSGD. HSGD carbons express extremely high activity for the removal of UB from HSA containing solutions (more than 100 mg of UB per 1 g of activated carbon).
View Article and Find Full Text PDFThe ability of albumin to bind drugs and other lipophilic organic acids is decreased in chronic renal failure by the accumulation of albumin-bound uraemic toxins such as hippuric acid, indoxyl sulphate and 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF). This furan acid is the most highly bound and is not removed by haemodialysis. The inhibitory effects of these three uraemic toxins on the interaction of three marker ligands sodium octanoate (for medium chain fatty acids), salicylic acid and phenol red (bilirubin site/site I) with albumin have been investigated by differential scanning microcalorimetry and flow microcalorimetry.
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