The effect of a mesenchymal stem cell conditioned medium fraction on morphological characteristics of hepatocytes in acetaminophen-induced acute liver failure: a preliminary study.

In our studies, it was shown that the effectiveness of the conditioned medium obtained by cultivating mesenchymal stem cells depends on the microenvironment conditions used to cultivate the cells. It was demonstrated that the conditioned medium obtained by culturing cells with low oxygen content (10%) has a much more pronounced protective effect. Protein compositions obtained from MSCs cultured under hypoxic (10% O hc-MSC) and normal (21% O nc-MSC) conditions were used to treat acute liver failure (ALF) induced in mice by acetaminophen injection.