Ionic Referencing in Surface Plasmon Microscopy: Visualization of the Difference in Surface Properties of Patterned Monomolecular Layers.

An approach for visualization of patterned monomolecular layers in surface plasmon microscopy (SPM) is suggested. The development of hidden image in SPM is achieved by referencing of images obtained in the presence of electrolytes with a high molar refraction of either anions or cations. A formation of diffuse layer near the charged surface areas leads to the redistribution of ions.

Plasmonic detection and visualization of directed adsorption of charged single nanoparticles to patterned surfaces.

It has recently been shown that surface plasmon microscopy (SPM) allows single nanoparticles (NPs) on sensor surfaces to be detected and analyzed. The authors have applied this technique to study the adsorption of single metallic and plastic NPs. Binding of gold NPs (40, 60 and 100 nm in size) and of 100 nm polystyrene NPs to gold surfaces modified by differently ω-functionalized alkyl thiols was studied first.

Detection and Quantification of Single Engineered Nanoparticles in Complex Samples Using Template Matching in Wide-Field Surface Plasmon Microscopy.

An ultrasensitive analytical method for direct detection of single nanoparticles in complex environment is described. The method relies on the wide-field surface plasmon microscopy (SPM). The suppression of matrix effects is achieved by image analysis based on the template matching.