Synthesis and Evaluation of Gelatin-Chitosan Biofilms Incorporating Zinc Oxide Nanoparticles and 5-Fluorouracil for Cancer Treatment.

In this study, a novel multifunctional biofilm was fabricated using a straightforward casting process. The biofilm comprised gelatin, chitosan, 5-fluorouracil (5-FU)-conjugated zinc oxide nanoparticles, and polyvinyl alcohol plasticized with glycerol. The 5-FU-conjugated nanoparticles were synthesized via a single-step co-precipitation process, offering a unique approach.

Construct Fluorescent Solid Lipid Nanoparticles from Bacterial Outer Membrane Vesicles to Study their Properties and Potential Applications as In Vivo and Invitro Imaging Agents.

In this study, the new solid lipid nanoparticles were created by combining fluorescent dye, fatty acid, lipid, and bacterial outer membranes. The synthesised particles were roughly 95-100 nm in size. Vero cells cultivated with these nanoparticles showed no cytotoxicity in 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT) assay.

Site-specific delivery of green tea coated aluminium magnesium silicate beads and studies on their effect against chicken coccidiosis.

In this report, the site-specific co-delivery of green tea/aluminium magnesium silicate (AMS) was reported and the specific target delivery was achieved orally. The new co-precipitation process was developed to synthesis the green tea/AMS hybrid complex and using energy-dispersive X-ray spectroscopy, Fourier-transform infrared spectroscopy and Raman confirmed its successful synthesis. The blood biocompatibility of the green tea/AMS was tested using chicken blood, and the compound is safe up to 500 mg/ml.

Cisplatin-loaded albumin nanoparticle and study their internalization effect by using β-cyclodextrin.

The present study with aim at enhancing the therapeutic and anti-cancer properties of cisplatin (CPT)-loaded bovine serum albumin (BSA) nanoparticles. The BSA nanoparticles containing CPT (CPT-BSANPs) were successfully prepared by the desolvation technique. The physicochemical characterization of the CPT-BSANPs were used by Fourier transformed infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and atomic force microscopy (AFM).

Studies on antimicrobial and wound healing applications of gauze coated with CHX-Ag hybrid NPs.

In this study, chlorhexidine (CHX)-silver (Ag) hybrid nanoparticles (NPs) coated gauze was developed, and their bactericidal effect and wound healing capacities were tested. A new method was developed to synthesise the NPs, wherein Ag nitrate mixed with sodium (Na) metaphosphate and reduced using Na borohydride. Finally, CHX digluconate was added to form the hybrid NPs.

Ketoconazole-conjugated ZnO nanoparticles based semi-solid formulation and study their impacts on skin disease.

In this study, the ketoconazole-conjugated zinc oxide (ZnO) nanoparticles were prepared in a single-step approach using dextrose as an intermediate compound. The physical parameters confirmed the drug conjugation with ZnO and their size was around 70-75 nm. The drug loading and drug release studies indicated that the -CHO group from the dextrose increase the drug loading up to 65% and their release kinetics were also studied.

Chlorhexidine-calcium phosphate nanoparticles - Polymer mixer based wound healing cream and their applications.

In this work, we developed a wound healing cream composed of two different polymers, namely chitosan and gelatin with chlorhexidine along with calcium phosphate nanoparticles. The physicochemical properties of the prepared cream were investigated based on SEM, EDX, Raman, FTIR and the results indicated that the cream contained gelatin, chitosan, calcium phosphate nanoparticles and chlorhexidine. The maximum swelling ratio studies indicated that the ratio was around of 52±2.

In ovo delivery of Newcastle disease virus conjugated hybrid calcium phosphate nanoparticle and to study the cytokine profile induction.

In this report, the hybrid calcium phosphate (CaP) nanoparticles were synthesized and functionalized with Newcastle disease virus (NDV). These nanoparticles were synthesized by a combination of co-precipitation and polymerization process and functionalized with amino propyl triethoxy silane before coupling to NDV. The 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide (MTT) assay of chicken spleen cells incubated with these nanoparticles indicated that, these particles did not exert any significant cytotoxicity.

Rapid determination of hydrogen peroxide produced by Lactobacillus using enzyme coupled rhodamine isocyanide/calcium phosphate nanoparticles.

A sensitive method for detecting hydrogen peroxide (H2O2) using rhodamine isocyanide incorporated calcium phosphate nanoparticles (Rho/CaP) was developed. The synthesized nanoparticles were characterized based on transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transformed infrared spectroscopy (FTIR), Raman spectroscopy, energy-dispersive X-ray spectroscopy (EDX) and X-ray diffraction patterns (XRD). To study the application, the nanoparticles were functionalized with horse radish peroxidase (HRP) based on aminopropyl triethoxy silane (APTES) and used as tools to detect H2O2.

Formulation of Newcastle disease virus coupled calcium phosphate nanoparticles: an effective strategy for oculonasal delivery to chicken.

In this report, calcium phosphate (CaP) nanoparticles were synthesized by continuous flow method using β-cyclodextrin (β-CD) as a medium and functionalized with amino propyl triethoxy silane (APTES). The blood biocompatibility of the nanoparticles was assessed using the whole blood haemolysis, erythrocytes haemolysis and erythrocyte aggregation tests. Based on the results, we found that the synthesized β-CD-CaP nanoparticles did not cause any remarkable toxic effect.

Development of antibiotic selection kit towards veterinary applications using glycine passivated magnetic particles.

Glycine functionalized (Gly/Fe3O4) and non-functionalized (Fe3O4) magnetic particles were synthesized in an autoclave and characterized by transmission electron microscopy (TEM), Fourier transformed infrared spectroscopy (FTIR), vibrating sample magnetometer (VSM), energy-dispersive X-ray spectroscopy (EDX), differential scanning calorimetry (DSC), X-ray diffraction patterns (XRD) and zeta potential. The size of the both these particles were in the range of 220-230 nm but the shape of the Gly/Fe3O4 particles was hexagonal in contrast to the spherical shape of Fe3O4 particles. The particle characterization tests confirmed that glycine was functionalized on the Gly/Fe3O4 particles, they were positively charged and possessed strong magnetic property.