Publications by authors named "Vishruta Pai"
Therapeutic strategy for efficiently targeting cancer cells needs an in-depth understanding of the cellular and molecular interplay in the tumor microenvironment (TME). TME comprises heterogeneous cells clustered together to translate tumor initiation, migration, and proliferation. The TME mainly comprises proliferating tumor cells, stromal cells, blood vessels, lymphatic vessels, cancer-associated fibroblasts (CAFs), extracellular matrix (ECM), and cancer stem cells (CSC).
View Article and Find Full Text PDFArticle Synopsis
- Natural polymeric hydrogels, particularly alginate/carboxymethylcellulose (Alg/CMC), are effective for creating 3D cell cultures, specifically for Huh-7 hepatocellular carcinoma cells using a freeze-thaw ionic crosslinking technique.
- The characterization of these hydrogels shows they have ideal properties like uniformity, appropriate pore size, viscoelasticity, and optimal degradation rates for supporting 3D culture.
- The study reveals that 3D spheroids reach a size of 250-300 μm by day 7, with gene expression analysis indicating significant upregulation of key proteins associated with cell interactions and maintaining epithelial characteristics during spheroid formation.