Premise Of The Study: Microsatellite markers were developed for (Polypodiaceae) to facilitate investigation of species boundaries between and its putative hybrid, , and potentially among other members of the Miocene-age species complex.
Methods And Results: Forty-eight primer pairs were designed from Illumina data and screened for successful amplification. Sixteen pairs were genotyped and evaluated for variability within and among three populations in North Carolina, Vermont, and New Hampshire.