Trimethoprim resistance of Escherichia coli in outpatients in Finland after ten years' use of plain trimethoprim.

Development of trimethoprim resistance among Escherichia coli collected from urine samples in three areas in Finland was studied in 1978-1984. Three different trends of development of resistance were found: in the Turku area resistance has increased evenly during 1978-1982 from 5.4% to 10.

Ampicillin concentrations in middle ear effusions in acute otitis media after administration of bacampicillin.

The penetration of ampicillin into middle ear effusions after oral administration of 800 mg bacampicillin to patients with acute otitis media was studied and compared with the serum levels. One hour after administration a mean (+/- SEM) concentration of 2.4 +/- 0.

Lysozyme activity and immunoglobulins in middle ear effusion fluid in acute purulent otitis media and in otitis media with effusion.

147 samples of punctured middle ear effusion fluid from cases of otitis media with effusion and 150 samples from patients with acute purulent otitis media were tested for lysozyme activity. In otitis media with effusion the concentration was 182.0 U/ml, in acute otitis 433.

Azidocillin and ampicillin concentrations in middle ear effusion.

The penetration of azidocillin and ampicillin into the middle ear effusion after oral administration was studied and compared with blood levels of the drugs. In acute otitis media one hour after a single oral dose of azidocillin (15 mg/kg) 1.56 +/- 0.

[Acidocillin concentration in middle ear exudate].

Acidocillin levels were determined in the middle ear exudates and serum of patients with acute exudative otitis media after oral administration of the drug. Acidocillin rapidly diffused exudate into the ear and its level in the exudate in 1 hour was 0-1.7 gamma/ml.

Binding of folic acid to serum proteins. V. Tritiated folic acid in the liver.

Sephadex G-200 chromatographies of the liver cell sap of rabbits, 1, 3 and 6 days after an injection of 3H-folic acid activity (FAA) into the circulation, were used in an attempt to study the binding of this labelled FAA to liver proteins. The labelled FAA was quickly accumulated in the liver, and in the cell sap it was grouped chromatographically to two maxima, which corresponded to the two maxima of microbiological FAA (L. casei).

Sensitivity of Haemophilus vaginalis (Corynebacterium vaginale) to oleandomycin and spectinomycin.

Haemophilus vaginalis is highly sensitive to oleandomycin. The MIC ranged from 0.035 to 0.

Gram-positive, motile, cluster-forming cocci as a cause of urinary infection.

One hundred and thirteen strains of motile, Gram-positive, catalase-positive, cluster-forming cocci were isolated from patients with urinary infection attending a private surgery. They constituted 1% of the total 11 302 positive cultures. The biochemical characteristics and the drug sensitivities of the strains are described.

Serum folic acid activity (L. casei) in Sephadex gel chromatography.

The folic acid activity (FAA) of the protein zone in DEAE Sephadex A-50 chromatography of normal serum showed the following distribution: most (about 40%) was eluted with protein zone IV (alpha-2-macroglobulin), about 33% with protein zones II-III (transferrin), and the balance with zone V (albumin). In healthy human subjects the variations were relatively small. The repeatability of the chromatography in an individual was good.

Blood group activities in water-soluble glycoproteins and in microsomal fractions of gastric mucosa in peptic ulcer and gastric cancer patients.

The existence of ABH blood group activities in water-soluble glycoproteins of human gastric mucosa, particularly in peptic ulcer and gastric cancer patients was studied. Special attention was paid to the sampling technique which would allow one to get a more complete picture of the total gastric mucosal area. An attempt was also made to localize the blood group substances in subcellular fractions.

The blood group activity of human gastric sulphoglycoproteins in patients with gastric cancer and normal controls.

Sulphoglycoproteins, isolated from human gastric juice, were observed to possess blood group activity, compatible with hosts' blood group in eighteen normal subjects and in nine patients with atrophic gastritis. The activities were of comparable magnitude with neutral glycoproteins of gastric juice as measured with haemagglutination inhibition test. The content of neutral glycoproteins is, however, about ten times that of sulphoglycoproteins and, it was thought, this would be the reason why the latter had been previously overlooked.