Influence of spermine and nitrogen deficiency on growth and secondary metabolites accumulation in Benth. cultured in a RITA temporary immersion system.

The effect of exogenous spermine (SPM) on shoots developing under nitrogen deficiency (ND) stress was evaluated. Shoots cultivated in a temporary immersion system were subjected to four experimental treatments: (1) control; (2) exogenous SPM; (3) ND; and (4) ND+SPM. Shoots were longer in the ND+SPM treatment (6.