Relationships between time use and obesity in a representative sample of Americans.

Objective: To provide a nationally representative analysis of time use in America for insight into behaviors associated with obesity.

Methods: This study utilized 28,503 observations of individuals aged 22 to 70 from the American Time Use Survey, a continuous cross-sectional survey on time use in America. Linear and logistic regressions were performed to analyze sociodemographic characteristics, determine activity participation levels and time spent in activities, understand nonlinear associations between activity time and BMI, and appreciate differences in activity timing between BMI categories.

Plasmodium falciparum erythrocytic stage parasites require the putative autophagy protein PfAtg7 for normal growth.

Analysis of the Plasmodium falciparum genome reveals a limited number of putative autophagy genes, specifically the four genes involved in ATG8 lipidation, an essential step in formation of autophagosomes. In yeast, Atg8 lipidation requires the E1-type ligase Atg7, an E2-type ligase Atg3, and a cysteine protease Atg4. These four putative P.

Survival and invasiveness of astrocytomas promoted by erythropoietin.

Object: The hypoxia-inducible pleiotropic hormone, erythropoietin (EPO), has recently been found to promote the development and survival of neurons and astrocytes. Since hypoxia has been implicated in the malignant progression of some human cancers, the authors investigated whether EPO signaling influenced the malignant properties of human astrocytoma cells.

Methods: Reverse transcriptase-polymerase chain reaction, Western blot analysis, and immunohistochemical studies were used to measure EPO and its receptor (EPOR).