Stepwise precipitation method of human blood lipoproteins for clinical routine.

A three-step precipitation method for separate measurement of the lipid and protein composition of the main serum lipoprotein classes has been developed. The consecutive procedure applying phosphotungstic acid, polyethylene glycol and dextran sulfate makes the analysis of chylomicron, VLDL, LDL, HDL2 and HDL3 possible. Because of simplicity and appropriate accuracy the method may be suitable for systematical use in clinical laboratories.

Accumulation of cholesterol ester in cultured smooth muscle cells of congenital hypercholesterolemic (WHHL) rabbits treated with normal and WHHL rabbit plasma LDL.

The DNA content, thymidine incorporation into DNA, and free and esterified cholesterol contents of cultured aortic smooth muscle cells (SMCs) of normal and WHHL rabbits were compared. In the first series of experiments, 5 groups of cultured normal rabbit aortic SMCs were compared: control cells and cells treated with LDL from normolipemic rabbit plasma (LDLN) and (DL from hypercholesterolemic rabbit plasma (LDLW), LDLN plus esterastin and LDLW plus esterastin. In the second series, the same groups of hereditable hypercholesterolemic WHHL rabbit aortic SMCs were compared.

Investigation of the interaction between Probon and indomethacin in rats.

The toxic side-effects and anti-inflammatory activity of combination treatment of Sprague-Dawley rats with indomethacin and Probon, a new type of analgesic, were investigated. Following four weeks of treatment with a combination of these two drugs, the characteristic gastrointestinal side-effect of indomethacin was markedly reduced. Mortality resulting from administration of the drug combination was significantly lower than that resulting from indomethacin treatment alone.

Effect of CH-1243, a pyrido (1,2-a) pyrimidine derivative on the elevated activity of lysosomal enzymes of rabbit aorta and liver in experimental atherosclerosis.

The effect of CH-123 (3-carbethoxy-6-methyl-1-9-(carboxy-methyl)-1-4-oxo-6,7,8,9-tetrahydro-4H-pyrid o(1,2a)pyrimidine) was investigated on the activity of 4 lysosomal enzymes: beta-glucuronidase, beta-galactosidase, N-acetyl-beta-glucosaminidase and acid phosphatase obtained from aortic smooth muscle and liver cells of rabbits. Animals were fed on a 2% cholesterol diet for 4 weeks and used an experimental atherosclerotic group. In drug-treated groups, after 4 weeks of cholesterol feeding the diet was changed to regular food and the animals were treated daily either with 50 mg/kg CH-123 or with 250 mg/kg Clofibrate.

Experimental models of atherosclerosis (the insudative theory).

Atherosclerosis, a disease of multifactorial origin, can be promoted or caused experimentally by a wide variety of methods. The leading role of altered endothelial permeability, as well as different experimental techniques for producing changes in permeability, have been discussed by selecting the insudative theory as a working hypothesis. An attempt has been made to prove that by altering the endothelial permeability with different types of injuries, atherosclerotic vascular damage can be provoked with or without hypercholesterolemia.

Nitrogen bridgehead compounds. Part 7: Synthesis of anti-atherosclerotic pyrido[1,2-a]pyrimidines.

The synthesis of 9-substituted-pyrido[1,2-a]pyrimidines are described. The new compounds exhibit antiatherosclerotic effects. The 3-ethoxycarbonyl-6-methyl-4-oxo-6,7,8,9-tetrahydro-4H-pyrido[1,2-a]pyrimidine-9-acetic acid (Chinoin-123) has the most favourable effects.

Effect of CH-123 on vascular cholesterol deposition in experimental atherosclerosis of rabbits.

The effect of CH-123, a new antiarteriosclerotic pyrido-1,2a-pyramide derivate was checked in experimental atherosclerosis of rabbits. Cholesterol deposition was found to continue after 4-week cholesterol feeding even after the cholesterol level had returned to normal. This allowed to study the action of drugs in a phase dominated by lipid apposition to the vascular wall.

[A new diet for inducing early experimental atherosclerosis in rabbits].

Authors by a new combined diet consisting of cholesterol vitamin D3 and sour cream produced clinico-biochemical changes characteristic for atherosclerosis in a very short period in rabbits. This diet caused severe lipoproteinaemia, hypercholesterinaemia and microscopis and macroscopic changes in the aortic wall. Authors assume, that this diet is suitable for producin atherosclerosis in a short period.

[Clinical use of prostaglandin antagonists in obstetrics].

The Department of Obstetrics and Gynecology in the Institute of Postgraduate Medical School (Budapest) reports on the treatment of 136 gravidities with the medicamentation of Prostaglandin-Antagonist.

[A new method for the study of flow of material into surviving blood vessel segments].

One of the most important questions arising in experiment on surviving segments of vessels is the question of flow of material into the vessel wall through the intima or adventitia. To make a distinction is important for the interpretation of results of in vitro experiments. By using a "tissuephil" tested already in practical surgery wound-adhesive free of any noxious effect, authors could form segments of vessels, having an only intimal or adventitial surface.