Publications by authors named "Vipsa Mehta"
Objectives: To increase testing capability for SARS-CoV-2 during a rapidly evolving public health emergency, we aimed to deploy a validated laboratory-developed real-time reverse transcription polymerase chain reaction (RT-PCR) diagnostic test for SARS-CoV-2 on an accelerated timeline and using reagent supply chains that were not constrained.
Methods: A real-time RT-PCR assay that detects the structural envelope ( gene of SARS-CoV-2 was developed and validated on the Roche cobas 6800 instrument platform with the omni Utility channel reagents, which performs automated nucleic acid extraction and purification, PCR amplification, and detection. analysis was performed for both inclusivity of all SARS-CoV-2 variants and cross reactivity with other pathogenic organisms.