How do we sleep? Towards physical requirements for space and environment while travelling.

To establish guidelines for sleeping space in vehicles, the sleeping postures of 189 participants are studied, 105 of them were asked to take the position in which they fall asleep and 84 have been asked to assume the position in which they lie most of the time. Seven percent slept on the stomach, 19% on the back and 74% on the side and 49% slept on the side with both legs flexed. For all participants a bed size of 200 × 90 cm will do.

Modeling passenger comfort in turboprop aircraft using objective measures.

Background: A quantitative comfort model will aid in evaluating comfort levels of various target groups before the actual flight of an airplane. However, constructing the model is always a challenge due to the complexity of the phenomenon.

Objectives: In this paper, we present quantitative comfort models to predict the (dis)comfort of passengers flying with turboprops based on objective measures.

Does a reclined backrest with less legroom meet the same comfort as a fixed backrest with 80 mm more leg room?

Background: In vehicles there is often limited space for seats. This might mean that reclining the back rest reduces the legroom. The second row in a cargo van has this problem and in this limited space an upright seat and a reclined seat with less legroom was developed and tested.

2D and 3D Self-Assembly of Fluorine-Free Pillar-[5]-Arenes and Perfluorinated Diacids at All-Aqueous Interfaces.

The interaction of perfluorinated molecules, also known as "forever chemicals" due to their pervasiveness, with their environment remains an important yet poorly understood topic. In this work, the self-assembly of perfluorinated molecules with multivalent hosts, pillar-[5]-arenes, is investigated. It is found that perfluoroalkyl diacids and pillar-[5]-arenes rapidly and strongly complex with each other at aqueous interfaces, forming solid interfacially templated films.

Anti-citrullinated histone monoclonal antibody CIT-013, a dual action therapeutic for neutrophil extracellular trap-associated autoimmune diseases.

Neutrophil extracellular traps (NETs) contribute to the pathophysiology of multiple inflammatory and autoimmune diseases. Targeting the NETosis pathway has demonstrated significant therapeutic potency in various disease models. Here, we describe a first-in-class monoclonal antibody (CIT-013) with high affinity for citrullinated histones H2A and H4, which inhibits NETosis and reduces tissue NET burden with significant anti-inflammatory consequences.

Recruiting participants for ergonomic research using self-reported stature and body mass.

Background: A valid distribution of key anthropometric parameters among participants is often a perquisite of ergonomics research.

Objective: In this paper, we investigated the accuracy of self-reported stature and body mass of the population in the Netherlands.

Methods: Data from 4 experiments was synthesized where in each experiment, participants self-reported their stature and body mass prior to being measured, of which they were not notified before.

Straightforward model construction and analysis of multicomponent biomolecular systems in equilibrium.

Mathematical modelling of molecular systems can be extremely helpful in elucidating complex phenomena in (bio)chemistry. However, equilibrium conditions in systems consisting of more than two components, such as for molecular glues bound to two proteins, can typically not be analytically determined without assumptions and (semi-)numerical models are not trivial to derive by the non-expert. Here we present a framework for equilibrium models, geared towards molecular glues and other contemporary multicomponent chemical biology challenges.

Integrated Single-Cell (Phospho-)Protein and RNA Detection Uncovers Phenotypic Characteristics and Active Signal Transduction of Human Antibody-Secreting Cells.

Single-cell technologies are currently widely applied to obtain a deeper understanding of the phenotype of single-cells in heterogenous mixtures. However, integrated multilayer approaches including simultaneous detection of mRNA, protein expression, and intracellular phospho-proteins are still challenging. Here, we combined an adapted method to in vitro-differentiate peripheral B-cells into antibody-secreting cells (ASCs) (i.

Measuring pressure distribution under the seat cushion and dividing the pressure map in six regions might be beneficial for comfort studies of aircraft seats.

Seat pressure maps are often used to evaluate comfort of the users. In this study, we explored the relationships between pressure maps and comfort/discomfort of users in aircraft seats with a focus on a new 6-division method on the pressure maps collected at the bottom of the cushions. An experiment was designed where three cushions with identical shapes but different stiffnesses were prepared.

Safety and efficacy of recombinant and live herpes zoster vaccines for prevention in at-risk adults with chronic diseases and immunocompromising conditions.

Compared with the general population, older adults with immune senescence and individuals who are immunocompromised (IC) due to disease or immunosuppressive therapy are at increased risk for herpes zoster (HZ) and its associated complications, which can be debilitating and life-threatening. Vaccination can be an effective strategy against HZ and studies have shown that HZ vaccination in IC individuals can elicit immune responses and provide protection from infection. Recently, the first approvals have been granted in the United States and the European Union for the recombinant HZ vaccine (RZV) in adults ≥ 18 years of age at risk of HZ due to immunodeficiency or immunosuppression.

Dense 3D pressure discomfort threshold (PDT) map of the human head, face and neck: A new method for mapping human sensitivity.

When designing wearables that interface with the human head, face and neck, designers and engineers consider human senses, ergonomics and comfort. A dense 3D pressure discomfort threshold map could be helpful, but does not exist yet. Differences in pressure discomfort threshold for areas of the head, neck and face were recorded, to create a 3D pressure discomfort threshold map.

Single-cell intracellular epitope and transcript detection reveals signal transduction dynamics.

To further our understanding of how biochemical information flows through cells upon external stimulation, we require single-cell multi-omics methods that concurrently map changes in (phospho)protein levels across signaling networks and the associated gene expression profiles. Here, we present quantification of RNA and intracellular epitopes by sequencing (QuRIE-seq), a droplet-based platform for single-cell RNA and intra- and extracellular (phospho)protein quantification through sequencing. We applied QuRIE-seq to quantify cell-state changes at both the signaling and the transcriptome level after 2-, 4-, 6-, 60-, and 180-min stimulation of the B cell receptor pathway in Burkitt lymphoma cells.

Cooperativity as quantification and optimization paradigm for nuclear receptor modulators.

Nuclear Receptors (NRs) are highly relevant drug targets, for which small molecule modulation goes beyond a simple ligand/receptor interaction. NR-ligands modulate Protein-Protein Interactions (PPIs) with coregulator proteins. Here we bring forward a cooperativity mechanism for small molecule modulation of NR PPIs, using the Peroxisome Proliferator Activated Receptor γ (PPARγ), which describes NR-ligands as allosteric molecular glues.

Development of a Soft Robotics Module for Active Control of Sitting Comfort.

Sitting comfort is an important factor for passengers in selecting cars, airlines, etc. This paper proposes a soft robotic module that can be integrated into the seat cushion to provide better comfort experiences to passengers. Building on rapid manufacturing technologies and a data-driven approach, the module can be controlled to sense the applied force and the displacement of the top surface and actuate according to four designed modes.

The Haptic Fidelity Framework: A Qualitative Overview and Categorization of Cutaneous-Based Haptic Technologies Through Fidelity.

After decades of research and development, haptic feedback is increasingly appearing in consumer products. While the prevalence of haptic feedback is increasing, the integration rarely offers increased fidelity to previous generations. We argue this is because of the tremendous complexity of successful haptic design engineering, but critically, also because of information saturation.

[Very early medical abortion, a termination of pregnancy before ultrasound evidence: something to aim for in the Netherlands?].

Objective: A very early medical abortion (VEMA) is an abortion early in pregnancy before the pregnancy is visible on an ultrasound. This treatment has been offered in several countries for many years. Our objective was to investigate whether VEMA is also desirable and can be carried out safely, in the Netherlands.

Supramolecular Enhancement of a Natural 14-3-3 Protein Ligand.

Rational design of protein-protein interaction (PPI) inhibitors is challenging. Connecting a general supramolecular protein binder with a specific peptidic ligand provides a novel conceptual approach. Thus, lysine-specific molecular tweezers were conjugated to a peptide-based 14-3-3 ligand and produced a strong PPI inhibitor with 100-fold elevated protein affinity.

Immune responses to the adjuvanted recombinant zoster vaccine in immunocompromised adults: a comprehensive overview.

Immunocompromised (IC) persons are at increased risk for herpes zoster (HZ) and its complications, mainly due to impairment of cell-mediated immunity (CMI). The adjuvanted recombinant zoster vaccine (RZV) demonstrated efficacy against HZ in autologous hematopoietic stem cell transplant (auto-HSCT) recipients and hematologic malignancy (HM) patients. We review immune responses to RZV in 5 adult IC populations, 4 of which were receiving multiple, concomitant immunosuppressive medications: auto-HSCT and renal transplant recipients, HM and solid tumor patients, and human immunodeficiency virus-infected adults.

Developments in work-related upper limb disorders (WRULD) amongst Dutch university students from 2004 to 2014.

Background: Former studies on work-related upper limb disorders (WRULD) within university education report substantial prevalence rates. In this study, developments in WRULD amongst students in the period 2004-2014 were investigated. Our findings can be a benchmark for future studies, in particular when there are major societal changes as in the case of the COVID-19 pandemic.

Identify dominant dimensions of 3D hand shapes using statistical shape model and deep neural network.

Hand anthropometry is one of the fundamentals of ergonomic research and product design. Many studies have been conducted to analyze the hand dimensions among different populations, however, the definitions and the numbers of those dimensions were usually selected based on the experience of the researchers and the available equipment. Few studies explored the importance of each hand dimension regarding the 3D shape of the hand.

Light-driven release of cucurbit[8]uril from a bivalent cage.

Temporal control over supramolecular systems has great potential for the modulation of binding and assembly events, such as providing orthogonal control over protein activity. Especially light controlled triggering provides unique entries for supramolecular systems to interface in a controlled manner with enzymes. Here we report on the light-induced release of cucurbit[8]uril (CB[8]) from a bivalent cage molecule and its subsequent activation of a proteolytic enzyme, caspase-9, that itself is unresponsive to light.

Human behaviour should be recorded in (dis)comfort research.

(Dis)comfort research that has no information on behaviour of the participants can be considered as incomplete, as major influencing factors could be missed. For (dis)comfort research it is important to capture factors such as context, task/activity, posture, movement, (distracting) stimuli and time, as these factors have influence on the experienced (dis)comfort. Recording the behaviour allows for better evaluation of and comparison between studies, contributing to an increase of scientific knowledge on (dis)comfort.

The effect of the standing angle on reducing fatigue among prolonged standing workers.

Background: Many occupations require workers to stand for prolonged periods, which can cause discomfort, pain and even injures. Some supermarkets in life provide a foot pad for checkout staff to let them stand on it at work, thereby reducing standing fatigue caused by standing for a long time. The inclined platform is the same as the foot pad mentioned above.

Effect of scent on comfort of aircraft passengers.

Background: Scents may influence the perceived comfort of an environment. There are only a few studies conducted on the relationship between scent and comfort in aircraft cabin.

Objectives: The goal of this research is to explore whether relationships between scents and perceived comfort can be found for passengers in an aircraft cabin.

Comfort and discomfort during smartphone use on a bed.

Background: The number of smartphone users is immense. People can do ever more with a smartphone. Smartphones are used everywhere, including in bed and on the sofa.