Challenges and opportunities in the analysis of raffinose oligosaccharides, pentosans, phytate, and glucosinolates.

In this paper, the status of the analytical technologies for assaying animal antinutritional compounds, such as raffinose oligosaccharides, pentosans, phytic acid, and glucosinolates, is reviewed in terms of selectivity, sensitivity, and sample throughput. The implementation of simplified sample preparation schemes, use of novel separation approaches, and alternate detector technologies are discussed. The challenges and opportunities posed by these assays are highlighted along with the recommendations for best analytical practices.

Design and synthesis of novel imidazole-substituted dipeptide amides as potent and selective inhibitors of Candida albicans myristoylCoA:protein N-myristoyltransferase and identification of related tripeptide inhibitors with mechanism-based antifungal activity.

A new class of antifungal agents has been discovered which exert their activity by blockade of myristoylCoA: protein N-myristoyltransferase (NMT; EC 2.1.3.

Rapid determination of logarithmic partition coefficients between n-octanol and water using micellar electrokinetic capillary chromatography.

Micellar electrokinetic capillary chromatography (MECC) was evaluated as a rapid screening tool for the determination of logarithmic partition coefficients between n-octanol-water (log P(OW)). The technique is performed by electrochromatographing a mixture of standards of known log P(OW). The logarithmic capacity factor of each standard was plotted against its log P(OW) to form a linear calibration curve for a given set of chromatographic conditions.

Rapid method for determination of 2-hydroxy-4-(methylthio)butanoic acid in poultry feeds by capillary isotachophoresis.

Capillary isotachophoresis, which involves the separation of charged species under an electric field, has been applied to the rapid determination of 2-hydroxy-4-(methylthio)butanoic acid at 0.04-0.50% concentration levels in corn/soy-based poultry feeds, using conductivity detection.

Sodium hypophosphite as reducing agent for determination of submicrogram quantities of mercury in animal feeds and manures.

The reduction of mercury in samples of dairy cattle and chicken feed rations and manures prepared by acid digestion for determination by flameless atomic absorption is rapidly, smoothly, and quantitatively effected by sodium hypophosphite. The reducing agent is air-stable, is effective over a wide range of mercury concentrations, and is useful in the presence of many mineral acids commonly used for wet digestion of these matrices. The accuracy and precision obtained in determinations with this reagent are equivalent to those obtained using stannous chloride at the same conditions.

Analysis of Animal Feed Ingredients and Soil Amendment Products Produced from Beef Cattle Manure for Selected Trace Metals Using Atomic Absorption Spectrophotometry.

Beef cattle manures have been converted to a water slurry and subjected to centrifugation, flocculution and drying to produce a silage replacement product (CI), a 20% protein powder from the centrifuge supernatant fluid (CII) and a soil amendment product (CIII). These products and the manure slurry were analyzed for their As, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb and Zn content by atomic absorption spectrophotometry. Different sample mineralization techniques, metal recovery efficiencies and matrix effects were investigated.