Case Report: Molecular Analyses of Cell-Cycle-Related Genes in Cortical Brain Tissue of a Patient with Rasmussen Encephalitis.

Rasmussen's encephalitis (RE) stands as a rare neurological disorder marked by progressive cerebral hemiatrophy and epilepsy resistant to medical treatment. Despite extensive study, the primary cause of RE remains elusive, while its histopathological features encompass cortical inflammation, neuronal degeneration, and gliosis. The underlying molecular mechanisms driving disease progression remain largely unexplored.

Optimization of the process of preparation of liver pâtés and omental fat of lambs fed with lipid cakes.

The aim of this work was to optimize the process of elaborating liver pâtés and omental lamb fat and to evaluate the quality of the products. Livers and fats were obtained from lambs fed with diets composed of corn and soybean meal that were partially replaced by cupuaçu, tucumã and palm kernel cake. To prepare the pâtés, livers were baked for 20 minutes at 100°C, weighed, seasoned, crushed, packaged and pasteurized.

Listeria monocytogenes in beef: a hidden risk.

Listeria monocytogenes in beef receives less attention compared to other pathogens such as Salmonella and Escherichia coli. To address this gap, we conducted a literature review focusing on the presence of L. monocytogenes in beef.

The sural-sparing pattern in clinical variants and electrophysiological subtypes of Guillain-Barré syndrome.

Background:  Guillain-Barré syndrome (GBS) is the most common cause of acute flaccid paralysis worldwide and can be classified into electrophysiological subtypes and clinical variants.

Objective:  This study aimed to compare the frequency of the sural-sparing pattern (SSP) in subtypes and variants of GBS.

Methods:  This retrospective cohort study analyzed clinical and electrophysiological data of 171 patients with GBS hospitalized in public and private hospitals of Natal, Rio Grande do Norte, Brazil, between 1994 and 2018; all cases were followed up by the same neurologist in a reference neurology center.

Biofilm-producing Escherichia coli O104:H4 overcomes bile salts toxicity by expressing virulence and resistance proteins.

We investigated bile salts' ability to induce phenotypic changes in biofilm production and protein expression of pathogenic Escherichia coli strains. For this purpose, 82 pathogenic E. coli strains isolated from humans (n = 70), and animals (n = 12), were examined for their ability to form biofilms in the presence or absence of bile salts.

In Silico Analysis of Shiga Toxin-Producing O157:H7 Strains from Presumptive Super- and Low-Shedder Cattle.

Cattle are the primary reservoir for STEC O157, with some shedding >10 CFU/g in feces, a phenomenon known as super-shedding (SS). The mechanism(s) responsible for SS are not understood but have been attributed to the environment, host, and pathogen. This study aimed to compare genetic characteristics of STEC O157 strains from cattle in the same commercial feedlot pens with SS or low-shedding (LS) status.

Optimizing the Antimicrobial Activity of Sodium Hypochlorite (NaClO) over Exposure Time for the Control of spp. In Vitro.

Fish is a nutritionally rich product; however, it is easily contaminated by pathogenic microorganisms, such as spp. Therefore, this study aimed to identify the best concentration of sodium hypochlorite (NaClO), exposure time, and water temperature that allow the most effective antimicrobial effect on the viable population of spp. Thus, Enteritidis ATCC 13076 and Schwarzengrund were exposed to different time frames, ranging from 5 min to 38.

Evaluation of the Composition of the Cholesterol, Tocopherols, β-Carotene and Fatty Acids Profile of the Liver Tissue of Male Water Buffaloes () Managed in Different Ecosystems of the Eastern Amazon.

The diet offered to animals has a great influence on the composition of tissues and, consequently, the quality. The objective of this study was to evaluate the influence of Amazonian ecosystems, in the dry and rainy periods of the year, on the composition of cholesterol, tocopherols, β-carotene and the fatty acid profile of the livers of water buffaloes () reared in the Eastern Amazon, in an extensive or intensive system. Total lipid content was influenced by the location and time of year ( < 0.

Lambs supplemented with Amazonian oilseed co-products: Meat quality and fatty acid profile.

The Amazon has a wide variety of oilseeds that generate a huge amount of co-products with potential for use in animal nutrition. The objective was to use alternative resources (oilseed cakes) in the feeding of lambs to assign a sustainable destination to this biomass, and evaluate its influence on the quality and fatty acid (FA) profile of the meat. Twenty-four lambs, male, castrated, crossbred Dorper × Santa Inês, weighing 30 ± 1.

Can Extraintestinal Pathogenic with Heat Resistance Profile Overcome Nonthermal Technologies?

Ultraviolet-C light-emitting diode (UVC-LED) and ultrasound (US) are two nonthermal technologies with the potential to destroy pathogens. However, little is known about their effectiveness in strains with a history of heat resistance. Thus, this study aimed to evaluate the phenotype and genotype of heat-resistant extraintestinal pathogenic (ExPEC) with heat resistance genes after the application of US, UVC-LED, and UVC-LED+US.

Influence of temperature and pH on induction of Shiga toxin in .

Shiga toxin-producing strains represent pathogenic group that is of concern in food production. The present study evaluated forty-eight isolates (11 with intact gene, while remaining isolates presented only -fragments) for Shiga toxin production. The four most expressive -producers (O26, O103, O145, and O157) were selected to evaluate effects of pH (3.

Evaluation of the quality of meat and carcasses from sheep fed diets containing three types of oils.

The objective of this research was to evaluate the quantitative and qualitative characteristics of the carcass and meat of lambs fed with different types of oil. Thirty male, uncastrated, mixed breed (Santa Inês × Dorper) sheep were used in this experiment and were distributed in random blocks with three treatments and 10 replicates per treatment, where each animal represents a replicated experimental unit. The three treatments were the following experimental diets: soybean oil in natura; soybean oil after use in frying, and palm oil ().

Efficacy of Quaternary Ammonium Compounds for Control of Individual and Mixed Cultures of with High- and Low-Quaternary Ammonium Compounds Resistance.

is a well-characterized micro-organism in scientific literature. Similarly, quaternary ammonium compounds (QACs) are historical sanitizers in food processing. However, the use of QACs has been questioned due to bacterial resistance in some studies.

Nutrient intake, digestibility, performance, carcass traits and sensory analysis of meat from lambs fed with co-products of Amazon oilseeds.

Introduction: The increase in availability and nutritional composition of oilseed co-products has made it essential to study the use of this biomass.

Methods: The objective of this work was to investigate the effects of including oilseed cakes on intake and digestibility, performance, carcass characteristics and meat sensory in feedlot lambs. Twenty-four crossbred Dorper × Santa Inês lambs, with initial body weight of 30 ± 1.

Methane emission, intake, digestibility, performance and blood metabolites in sheep supplemented with cupuassu and tucuma cake in the eastern Amazon.

The use of co-products as a feed supplement for ruminants makes livestock sustainable and optimizes the use of available areas and animal performance. Furthermore, when cakes are used, the residual fat composition can influence ruminal metabolism and methane (CH) production. This study aimed to assess the effects of a diet containing cupuassu (CUP; ) and tucuma (TUC; .

Phenolic Compounds in Bacterial Inactivation: A Perspective from Brazil.

Phenolic compounds are natural substances that are produced through the secondary metabolism of plants, fungi, and bacteria, in addition to being produced by chemical synthesis. These compounds have anti-inflammatory, antioxidant, and antimicrobial properties, among others. In this way, Brazil represents one of the most promising countries regarding phenolic compounds since it has a heterogeneous flora, with the presence of six distinct biomes (Cerrado, Amazon, Atlantic Forest, Caatinga, Pantanal, and Pampa).

Mineral Content of Liver of Buffaloes () Reared in Different Ecosystems in the Eastern Amazon.

This study aimed to evaluate the influence of different production ecosystems, three in native and cultivated pastures (extensive), at two seasons of the year (dry and rainy), and one in confinement (intensive) in the Eastern Amazon, on the mineral content of buffalo liver raised on these ecosystems. Twelve male buffalo ( = 12), aged between 24 and 36 months, slaughtered in commercial slaughterhouses, were used in each of the ecosystems considered: Marajó; Lower Amazon; Cultivated Pasture, and in confinement system, Pará, Brazil. Approximately 5 g of liver was collected, stored and frozen until lyophilization.

Muscle mineral profile of water buffaloes () reared in different production systems of the Brazilian Eastern Amazon.

Healthy food must have an adequate balance of macroelements, such as calcium or phosphorus and, microelements, such as iron, copper. This study aimed to evaluate the influence of three extensive systems, during the dry and rainy seasons, and an intensive (feedlot) system in the Eastern Amazon, on the muscle mineral profile of water buffaloes. In total, 12 male buffaloes, aged between 24 and 36 months, slaughtered in commercial slaughterhouses, were used in each of the systems considered: Marajó island, Santarém, Nova Timboteua, and a feedlot.

Heat-resistant and biofilm-forming Escherichia coli in pasteurized milk from Brazil.

Escherichia coli harboring a transmissible locus of stress tolerance (tLST) and the ability to form biofilms represent a serious risk in dairy production. Thus, we aimed to evaluate the microbiological quality of pasteurized milk from two dairy producers in Mato Grosso, Brazil, with a focus on determining the possible presence of E. coli with heat resistance (60 °C/6 min), biofilm-forming potential phenotypes and genotypes, and antimicrobial susceptibility.

Genomic features and heat resistance profiles of Escherichia coli isolated from Brazilian beef.

Aims: Characterize Escherichia coli and E. coli -producing (STEC) isolates from Brazilian beef to determine heat resistance and the presence of the transmissible locus of stress tolerance (tLST).

Methods And Results: Twenty-two STEC previously isolated from beef and characterized as STEC by PCR were subjected to different heat survival challenges (60°C and 71°C).

Feed intake, emission of enteric methane and estimates, feed efficiency, and ingestive behavior in buffaloes supplemented with palm kernel cake in the Amazon biome.

The use of palm kernel cake as an alternative to conventional ingredients, due to the presence of residual fat, can also reduce methane emissions. The objective of the study was to evaluate, in two different experiments, the effects of palm kernel cake supplementation on feed intake, enteric methane production and estimates, and the ingestive behavior of buffaloes in the Amazon biome. In experiment 1, to evaluate feed intake, methane production, and feed efficiency, 20 crossbred females, dry and empty, with a mean age of 34 months and an initial body weight of 514 ± 69 kg, were supplemented with palm kernel cake for 60 days.

Genomic analysis of variant strains isolated from bovine in the state of Mato Grosso, Brazil.

The species variant ( var. ) is associated with tuberculosis, mainly in cattle and buffaloes. This pathogen has the potential to infect other mammals, including humans.