Publications by authors named "Vinicius Carvalho Pereira"
Antimicrobial and antibiofilm effect of promethazine on bacterial isolates from canine otitis externa: an in vitro study.
Microb Pathog
November 2024
Article Synopsis
- Otitis externa is a condition in dogs where the external ear canal becomes inflamed, usually due to bacterial infections, and this study focuses on testing promethazine's effectiveness against it.
- * The research evaluated how promethazine affects bacterial growth and biofilm formation over 120 hours, determining its minimum inhibitory concentrations (MIC) and minimum biofilm eradication concentration (MBEC).
- * Results showed that promethazine significantly reduced biofilm growth and could be a promising treatment for recurring bacterial otitis in dogs, highlighting the benefits of repurposing existing drugs to combat antimicrobial resistance.
Article Synopsis
- The article investigates the use of carbonyl cyanide m-chlorophenyl hydrazone (CCCP) as an efflux pump inhibitor to combat antimicrobial resistance and biofilm formation.
- It found that CCCP significantly reduces biofilm biomass and enhances the effectiveness of antibiotics against biofilms when used at certain concentrations (128 µM and 512 µM).
- CCCP also impacts the production of virulence factors, decreasing protease production and increasing siderophore release, indicating its potential as a therapeutic aid in managing infections related to biofilms.
To evaluate the action of promethazine, fluoxetine and carbonyl cyanide 3-chlorophenylhydrazone as efflux pump inhibitors (EPIs) against multidrug-resistant . The effect of the compounds was evaluated in planktonic cells and bacterial biofilms. Accumulation tests were performed with ethidium bromide to prove their action as EPIs.
View Article and Find Full Text PDFThis study evaluated the antibiofilm activity of promethazine, deferiprone, and Manuka honey against Staphylococcus aureus and Pseudomonas aeruginosa in vitro and ex vivo in a wound model on porcine skin. The minimum inhibitory concentrations (MICs) and the effects of the compounds on biofilms were evaluated. Then, counting colony-forming units (CFUs) and confocal microscopy were performed on biofilms cultivated on porcine skin for evaluation of the compounds.
View Article and Find Full Text PDFThis study evaluated the effect of the iron chelator deferiprone (DFP) on antimicrobial susceptibility and biofilm formation and maintenance by . Planktonic susceptibility to DFP alone and in combination with antibiotics was evaluated by broth microdilution and biofilm metabolic activity was determined with resazurin. DFP minimum inhibitory concentration (MIC) range was 4-64 µg/mL and in combination reduced the MIC for amoxicillin/clavulanate and meropenem.
View Article and Find Full Text PDFEx vivo wound model on porcine skin for the evaluation of the antibiofilm activity of polyhexamethylene biguanide and ciprofloxacin.
Lett Appl Microbiol
March 2023
This study aimed to standardize the use of an ex vivo wound model for the evaluation of compounds with antibiofilm activity. The in vitro susceptibility of Staphylococcus aureus ATCC 29213 and Pseudomonas aeruginosa ATCC 27853 to ciprofloxacin and polyhexamethylene biguanide (PHMB) was evaluated in planktonic and biofilm growth. The effects of ciprofloxacin and PHMB on biofilms grown on porcine skin explants were evaluated by colony-forming unit (CFU) counting and confocal microscopy.
View Article and Find Full Text PDFThe present study evaluated the general welfare state of two strains of transgenic goats bred in a region with a hot and humid tropical climate. Nine females were used, being three transgenic for human lysozyme (hLZ group), three transgenic for human glucocerebrosidase (hGCase group), and three non-transgenic (control group). The temperature and humidity index (THI) were recorded during the morning, afternoon, and evening.
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