SNARE protein SNAP25 regulates the chloride-transporter KCC2 in neurons.

Inhibitory synaptic neurotransmission mediated by GABA requires a low concentration of chloride ions (Cl) in neurons, which is established and maintained by the potassium-chloride co-transporter 2 (KCC2). While KCC2-interacting proteins are known to regulate KCC2 protein level and function, specific KCC2-interacting partners are still being identified and characterized. We asked whether SNAP25, an integral component of the SNARE-complex and a novel KCC2 interactor, regulates KCC2 protein and function in mice.