Publications by authors named "Vincent Vandenbroucke"
Decoupling cell formation from recombinant protein synthesis is a potent strategy to intensify bioprocesses. Escherichia coli strains with mutations in the glucose uptake components lack catabolite repression, display low growth rate, no overflow metabolism, and high recombinant protein yields. Fast growth rates were promoted by the simultaneous consumption of glucose and glycerol, and this was followed by a phase of slow growth, when only glucose remained in the medium.
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- Isogenic cell populations can adapt to stress by switching to different phenotypes, but this variability is problematic for applications like bioproduction and synthetic biology.
- The study investigates how various systems (like bacteria and yeast) diversify under stress, revealing that the fitness cost of switching phenotypes correlates with population dynamics.
- A stochastic model identifies three diversification patterns—constrained, dispersed, and bursty—based on switching costs, and a tool called Segregostat allows for better control over these patterns for more predictable cellular behavior.
Different expression vectors are available for the effective production of recombinant proteins by bacterial populations. However, the productivity of such systems is limited by the inherent noise of the gene circuits used for the synthesis of recombinant products. An extreme case of cell-to-cell heterogeneity that has been previously reported for the ara- and lac-based expression systems in E.
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