Development of novel isothermal-based DNA amplification assay for detection of pig tissues in adulterated meat.

For the first time, we describe an innovative polymerase spiral reaction (PSR) assay for the rapid, simple, and accurate detection of pig tissues or pork in adulterated meat including heat-treated and processed ones. The PSR assay specifically targeting the mitochondrial cytochrome b () gene of the pig was successfully optimized permitting assay results in 65 min time. The developed detection method was 100% specific amplifying only the gene and displaying negative results with all the tested non-pork meats.