Structural characterization of novel hydrolytic and oxidative degradation products of acalabrutinib by LC-Q-TOF-MS, H/D exchange and NMR.

The drug substance, acalabrutinib was subjected to hydrolytic (acid, base and neutral) and oxidative stress degradation as per ICH recommendations. Degradation products (DPs) generated from the drug substance were separated on a Shimadzu Shim-pak C-8 column utilizing a mobile phase composed of methanol: acetonitrile (90:10 v/v) and ammonium acetate buffer (10 mM, pH 3.80) in a gradient elution mode.

Molecular encapsulation of andrographolide in 2-hydroxypropyl--cyclodextrin cavity: synthesis, characterization, pharmacokinetic and antiviral activity analysis against SARS-CoV-2.

In present investigation, AND-2-HyP--CYD (Andrographolide-2-Hydroxypropyl--cyclodextrin) complex was synthesized and characterized for antiviral and pharmacokinetic profile. The linear host-guest relation suggested synthesis of a 1:1 complex of AND with 2-HyP--CYD by inclusion mode. The Kc, stability constant of the two phase system of AND with 2-HyP--CYD computed to be 38.