Double-stranded RNA mediated knockdown of sucrase gene induced mortality and reduced offspring production in Aphis gossypii.

The importance of gut sucrase in maintaining osmotic equilibrium and utilizing phloem contents as a carbon source has been widely investigated and proven in sap-sucking insects. In the present study, silencing of Aphis gossypii sucrase1 (Agsuc1) was carried out by double-stranded RNA (dsRNA), which would be lethal to it due to disruption of osmotic balance. The dsRNA corresponding to Agsuc1 was synthesized by two methods, i.

Diversity of transgenes in sustainable management of insect pests.

Insecticidal transgenes, when incorporated and expressed in plants, confer resistance against insects by producing several products having insecticidal properties. Protease inhibitors, lectins, amylase inhibitors, and chitinase genes are associated with the natural defenses developed by plants to counter insect attacks. Several toxin genes are also derived from spiders and scorpions for protection against insects.

Comparison of phenotypic and genotypic frequency of phosphine resistance in select field populations of Tribolium castaneum from India.

Background: Tribolium castaneum causes substantial damage to stored grains, leading to economic losses. The present study evaluates phosphine resistance in adult and larval stages of T. castaneum from north and northeast India, where continuous and long-term phosphine use in large-scale storage conditions intensifies resistance, posing risks to grain quality, safety, and industry profitability.

Differential Activities of Antioxidant Enzymes, Superoxide Dismutase, Peroxidase, and Catalase vis-à-vis Phosphine Resistance in Field Populations of Lesser Grain Borer () from India.

Susceptibility to phosphine was compared in 15 populations of lesser grain borer () collected from grain storage godowns across India. A high level of resistance to phosphine was noticed in collected from northern India compared to those collected from northeastern regions of India. The median lethal concentration values varied from 0.

Leaf Damage Based Phenotyping Technique and Its Validation Against Fall Armyworm, (J. E. Smith), in Maize.

Globally, maize is an important cereal food crop with the highest production and productivity. Among the biotic constraints that limit the productivity of maize, the recent invasion of fall armyworm (FAW) in India is a concern. The first line of strategy available for FAW management is to evaluate and exploit resistant genotypes for inclusion in an IPM schedule.

Biology and biometric characteristics of Spodoptera frugiperda (Lepidoptera: Noctuidae) reared on different host plants with regard to diet.

Background: The fall armyworm (FAW) Spodoptera frugiperda (J. E. Smith), native to the Americas, is a new invasive pest that was reported in India for the first time in May 2018.

Sex Biased Variance in the Structural and Functional Diversity of the Midgut Bacterial Community of Last Instar Larvae of Pectinophora gossypiella (Lepidoptera: Gelechiidae).

Elucidating the midgut bacterial diversity in an important cotton bollworm Pectinophora gossypiella can be a stepping stone in understanding the possible role of midgut bacteria in field evolved resistance against Bt cotton as well as to commonly used insecticides. Present study targeted metagenomics of 16S rRNA V3-V4 region to understand the influence of sex, if exists, in community diversity of gut microbes vis a vis their function in pink bollworm larvae. The results of the present study revealed that Proteobacteria, Firmicutes, and Actinobacteria were the predominant phyla in the midgut of pink bollworm.