Evaluation of the presence of the premaxillary-maxillary suture in growing patients: A retrospective CBCT study.

Background: The premaxillary-maxillary suture (PMS) is related to midface development, but its exact closure period remains undefined. Some authors consider the premaxilla as an independent bone with potential for orthopaedic stimulation, while others see it as a phylogenetic vestige.

Objective: To determine the prevalence of the premaxillary-maxillary suture (PMS) in cone beam computed tomography (CBCT) images of patients aged 6 to 16years, according to age and sex, treated at the Dental Clinic of the Pontificia Universidad Católica de Chile's Oral Imaging Service between 2015 and 2023.

Co-occurrence of the cephalosporinase cepA and carbapenemase cfiA genes in a Bacteroides fragilis division II strain, an unexpected finding.

Background: Bacteroides fragilis, an anaerobic gut bacterium and opportunistic pathogen, comprises two genetically divergent groups (or divisions) at the species level. Differences exist both in the core and accessory genomes and the beta-lactamase genes, with the cephalosporinase gene cepA represented only in division I and the carbapenemase gene cfiA only in division II.

Methods: Multidrug resistance in a clinical B.

Comparison of digitally assessed quality of posterior crown preparations performed with and without previous practice on patient-specific three-dimensional-printed teeth models.

Introduction: Three-dimensional (3D)-printing technology can provide customizable simulations, but its effects on patient care quality have not been well studied. This study aimed to assess the impact of practicing with patient-specific 3D-printed teeth models on the quality of patients' dental preparations performed by students transitioning to clinical training. Accordingly, the quality of posterior crown preparations was evaluated by objectively analyzing digital scans and grades in two groups: the study group, which practiced beforehand with patient-specific 3D-printed teeth models, and the control group, which did not practice with these models.

Clinical, microbiological, and molecular characterization of pediatric invasive infections by in Spain in a context of global outbreak.

Unlabelled: In December 2022, an alert was published in the UK and other European countries reporting an unusual increase in the incidence of infections. Our aim was to describe the clinical, microbiological, and molecular characteristics of group A invasive infections (iGAS) in children prospectively recruited in Spain (September 2022-March 2023), and compare invasive strains with strains causing mild infections. One hundred thirty isolates of causing infection (102 iGAS and 28 mild infections) were included in the microbiological study: typing, antimicrobial susceptibility testing, and sequencing for core genome multilocus sequence typing (cgMLST), resistome, and virulome analysis.

Focusing on Infections: Distribution, Antimicrobial Susceptibilities and Phylogeny.

The immunosuppression conditions and the presence of medical devices in patients favor the infections. However, the features of this aerobic actinomycete have been little explored. Strains ( = 164) were characterized with 16S rDNA and A1 genes to define their phylogenetic relationships, and subjected to broth microdilution to profile the antimicrobial susceptibilities of species that caused infections in Spain during the 2005-2021 period.

Exploring the genetic background of the botulism neurotoxin BoNT/B2 in Spain.

To determine whether the neurotoxin BoNT/B2 causing botulism in Spain is clonal, the genetic diversity and phylogenetic relationships of from food-borne episodes and infant cases of the condition were explored. The botulinum toxin gene () subtype, the variable region of the flagellin gene (VR), and a seven-gene multi-locus sequence type were examined by sequencing 37 BoNT-positive cultures obtained over the period 2010 to 2022. Out of 37 botulism events, 16 food-borne episodes and 16 infant cases were associated with 2.

National Surveillance of Tetracycline, Erythromycin, and Clindamycin Resistance in Invasive : A Retrospective Study of the Situation in Spain, 2007-2020.

Background: This work reports on antimicrobial resistance data for invasive in Spain, collected by the 'Surveillance Program for Invasive Group A ', in 2007-2020.

Methods: typing was determined by sequencing. Susceptibility to penicillin, tetracycline, erythromycin, and clindamycin was detined via the E-test.

Characterization of group A beta-haemolytic streptococcus with mucoid phenotype isolated in a tertiary hospital.

Introduction: The objective of this study is to characterize Streptococcus pyogenes isolates with a mucoid phenotype and to compare them with non-mucoid isolates obtained between April and August 2016.

Material And Methods: Identification and antimicrobial susceptibility were performed in all isolates. The emm type and exotoxin genes speA, speB, speC, speF, speG, speH, speJ, speZ and ssa were analyzed.

Students' and tutors' perceptions of a deliberate simulated practice using patient-specific virtual and three-dimensional printed teeth models: A pilot study.

Objective: This pilot study aimed to investigate the perceptions of dental students and their tutors of a deliberate simulated practice using patient-specific virtual and three-dimensional (3D) printed teeth models. This is before they perform their first indirect posterior tooth restoration on their patients.

Methods: Seventy-eight fourth-year dental students from the 2021 Comprehensive Clinic I course at the University of the Andes, Chile, were invited to participate in a deliberate practice protocol.

Identification and antimicrobial susceptibility of and other unusual clinical isolates in Spain.

Two hundred and eighty-six isolates from human clinical samples were identified between 1996 and 2019 as belonging to 8 families, 19 genera and 88 species of . The most identified genera were (182 strains from 45 species), (29 strains, 5 species), (21 strains, 6 species) and (18 strains, 5 species). The rest of the identified genera (15) contained 27 species with 36 isolates.

Invasive Streptococcus pyogenes disease in Spain: a microbiological and epidemiological study covering the period 2007-2019.

The aim of this study is to present the first nationwide microbiological and epidemiological study of invasive group A Streptococcus (iGAS) disease in Spain. One thousand eight hundred ninety-three iGAS isolates were analyzed over 2007-2019. emm typing was performed by sequencing the gene's variable 5' end, exotoxin genes were identified by PCR, and antimicrobial susceptibility explored via the E test and disk diffusion.

Genomic Background and Phylogeny of A-Positive Strains Resistant to Meropenem-EDTA.

Background: shows high antimicrobial resistance (AMR) rates and possesses numerous AMR mechanisms. Its carbapenem-resistant strains (metallo-β-lactamase A-positive) appear as an emergent, evolving clade.

Methods: This work examines the genomes, taxonomy, and phylogenetic relationships with respect to other genomes of two strains (CNM20180471 and CNM20200206) resistant to meropenem+EDTA and other antimicrobial agents.

Characterization of group A beta-haemolytic streptococcus with mucoid phenotype isolated in a tertiary hospital.

Introduction: The objective of this study is to characterize Streptococcus pyogenes isolates with a mucoid phenotype and to compare them with non-mucoid isolates obtained between April and August 2016.

Material And Methods: Identification and antimicrobial susceptibility were performed in all isolates. The emm type and exotoxin genes speA, speB, speC, speF, speG, speH, speJ, speZ and ssa were analyzed.

Effect of macro-design in the primary stability of short and extra-short implants using resonance frequency analysis. An ex vivo study.

Objective: This study aimed to determine the effect of macro design in the primary stability of short and extra-short implants using resonance frequency analysis (RFA).

Material And Methods: On an ex-vivo model using pig's ribs, we inserted 80 short and extra-short dental implants (20 implants per brand): Biohorizons®(B) 4.6 × 6mm; Intralock®(I) 4.

Molecular Characterization and Antimicrobial Susceptibilities of Species Isolated from the Soil; A Comparison with Species Isolated from Humans.

species, one of the most predominant Actinobacteria of the soil microbiota, cause infection in humans following traumatic inoculation or inhalation. The identification, typing, phylogenetic relationship and antimicrobial susceptibilities of 38 soil strains from Lara State, Venezuela, were studied by 16S rRNA and (subunit B of topoisomerase II) genes, multilocus sequence analysis (MLSA), whole-genome sequencing (WGS), and microdilution. The results were compared with those for human strains.

gen. nov., sp. nov., a member of order , isolated from human blood.

The taxonomic position of an unknown bacterial strain designated CNM695-12, isolated from the blood of an immunocompromised subject, was investigated via phenotypic, chemotaxonomic, genotypic and genomic analyses. Bacterial cells were determined to be Gram-stain-negative bacilli, aerobic, non-motile and non-spore-forming. The strain showed catalase activity but no oxidase activity.

Dynamics of a Sporadic Nosocomial Complex Population.

Our objective was to improve current knowledge of sporadic (Spo) nosocomial (Acb) complex populations, and thus better understand the epidemiology of Spo and endemoepidemic (EE) strains. Between 1999 and 2010, 133 isolates of Spo Acb complex were obtained from a single hospital. Species were identified by B-PCR, and via B- and B-sequencing.

Characterization of Streptococcus pyogenes from Animal Clinical Specimens, Spain.

Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds.

spp. isolated from human and environmental samples in Spain: detection of 11 new species.

One hundred thirty-six isolates, 88 human and 48 environmental, that met the requirements to belong to the genus were identified using a polyphasic taxonomic approach known as 16S rRNA plus phenotypic traits. Thirty-seven species were identified; some had not been previously reported from clinical samples. The main species were (13 isolates), (11), (9) and (8).

Molecular characterization of invasive Streptococcus dysgalactiae subsp. equisimilis. Multicenter study: Argentina 2011-2012.

Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen.

Epidemiology and susceptibility to antimicrobial agents of the main Nocardia species in Spain.

Objectives: The aims of this study were to explore the clinical distribution, by species, of the genus Nocardia and to assess the antimicrobial susceptibilities of the 10 most prevalent species identified in Spain.

Methods: Over a 10 year period (2005-14), 1119 Nocardia strains were molecularly identified and subjected to the Etest. The distribution and resistance trends over the sub-periods 2005-09 and 2010-14 were also examined.

Resistance gene pool to co-trimoxazole in non-susceptible Nocardia strains.

The soil-borne pathogen Nocardia sp. causes severe cutaneous, pulmonary, and central nervous system infections. Against them, co-trimoxazole (SXT) constitutes the mainstay of antimicrobial therapy.

Endemic and epidemic Acinetobacter baumannii clones: a twelve-year study in a tertiary care hospital.

Background: Nosocomial outbreaks of multidrug-resistant Acinetobacter baumannii are of worldwide concern. Using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), and multiple locus variable number tandem repeat sequence (VNTR) analysis (MLVA), the present work examines the genetic diversity of the endemic and epidemic A. baumannii clones isolated in a single hospital over a twelve-year period.

Increase in isolation of Burkholderia contaminans from Spanish patients with cystic fibrosis.

Species of the Burkholderia cepacia complex are associated with opportunistic infection in patients with cystic fibrosis. For years now, B. multivorans and B.