Low to moderate doses of cycloheximide had a stimulatory effect on interferon production in rabbit kidney cell cultures treated with double-stranded polyinosinate-polycytidylate (poly I:poly C). A very marked stimulation occurred in the presence of a dose of cycloheximide inhibiting amino acid incorporation into total cellular protein by about 75%. Higher doses of cycloheximide caused a shift in interferon release towards later intervals and a gradual decrease in the overall degree of stimulation.
View Article and Find Full Text PDFPolyribocytidylate-(3)H-polyriboinosinate (rC-(3)H:rI) enters cultured rabbit kidney cells from the surrounding medium within (1/2) hr after exposure. Grains are found in the cytoplasm, nucleus, and nucleolus. At 2 hr, grains are localized predominantly over the nucleolar regions.
View Article and Find Full Text PDFRabbit kidney cell cultures stimulated with either double-stranded polyinosinate-polycytidylate (poly I:poly C) or with ultraviolet-irradiated Newcastle disease virus (UV-NDV) produce two types of interferon response, designated "early" and "late," respectively. The early response is suppressed by inhibitors of RNA or protein synthesis and is therefore thought to represent de novo synthesis of interferon. Circumstantial evidence suggested that this interferon response is regulated by a translation control mechanism.
View Article and Find Full Text PDFArch Gesamte Virusforsch
December 1970
Chick embryo cells became more sensitive to the action of interferon the longer they remained in culture. This phenomenon was found even before confluency had been reached. The relative insensitivity of newly seeded cells was not due to a loss of receptors.
View Article and Find Full Text PDFInjection of mice with two interferon inducers, Newcastle disease virus or statolon, 20 hours after inoculation with Plasmodium berghei sporozoites, prevented or delayed the development of detectable malarial parasitemia and death.
View Article and Find Full Text PDFJ Bacteriol
December 1966
Vilcek, Jan (New York University School of Medicine, New York, N.Y.), and John H.
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