Publications by authors named "Viktoryia Kulikouskaya"

Article Synopsis
  • * The treatment did not alter the functional groups of the polysaccharides but did change their monosaccharide composition and morphology, enhancing their anti-photoaging properties.
  • * The most effective polysaccharide, DSFP-45, significantly increased cell viability and collagen production while reducing inflammatory cytokines in UVB-irradiated human skin cells, indicating potential for new anti-photoaging drugs or functional foods from seaweed.
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Pseudomonas species are among the main pathogens causing rainbow trout infections. The present study provides a simple, green, sustainable, and rapid technique to synthesize of biogenic alginate-capped silver nanoparticles (Alg-Ag NPs) suitable for the treatment of Pseudomonas infections. It has been shown that the mechanism (aggregative or autocatalytic) of Alg-Ag NPs formation depended on Alg concentration and the heating approach used.

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Surface morphology affects cell attachment and proliferation. In this research, different films made of biodegradable polymers, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate--3-hydroxyvalerate) (PHB--HV), containing different molecular weights, with microstructured surfaces were investigated. Two methods were used to obtain patterned films-water-assisted self-assembly ("breath figure") and spin-coating techniques.

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Implant-related infection is one of the serious problems in regenerative medicine. Promising approach to overcome the problems caused by bacterial growth on the medical implants is their modification by bioactive coatings. A versatile technique for designing multilayer films with tailored characteristics at the nanometer scale is layer-by-layer assembly.

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Article Synopsis
  • - The study developed blend films using poly (vinyl alcohol) (PVA) and varying concentrations of chitosan oligosaccharides (COS), resulting in films with a brown hue and notable changes in their properties.
  • - FTIR spectroscopy and thermal analysis indicated that a 5.0 wt% COS concentration enhanced hydrogen bonding, crystallinity, and water evaporation in the films, improving their mechanical stability compared to the control film.
  • - The films demonstrated excellent antimicrobial activity when loaded with ciprofloxacin HCl and were found to be biocompatible, suggesting potential applications in drug delivery systems.
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There are few studies on seaweed polysaccharides with UV/HO treatment, so the aim of this study was to evaluate the effects of UV/HO treatment on physicochemical properties and RAW 264.7 cells responses of polysaccharides from Sargassum fusiforme (PSF). Results showed that the contents of reducing sugar and sulfate in PSF with UV/HO treatment for 2 h increased by 202.

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The study aimed to study the effects on structural characteristics and anti-inflammatory activities of algal sulfated polysaccharides isolated from Gracilaria lemaneiformis (GLP) after a combined treatment of UV irradiation (average irradiance of 6500 mJ/cm) and HO (50 mmol/L) for various time periods up to 60 min. After a 30-min treatment, the molecular weight and particle size of GLP was decreased by 15 and 2.6 fold, respectively with small but significant decrease in the contents of total sugars, uronic acids and proteins.

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The design of novel wound dressings for chronic wound treatment is still of great importance. One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing. In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated.

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A sulfated polysaccharide extracted from Gracilaria lemaneiformis (GLP) with a prominent effect in regulating lipid metabolism was isolated. The molecular weight was 31.5 kDa and it was composed mainly of galactose, glucose and xylose.

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Article Synopsis
  • The study focused on creating ultrathin poly(L-lactic acid) (PLLA) films using a spin-coating technique and examined their physical properties like thickness and transparency.
  • By adjusting polymer concentration and humidity, researchers were able to control the film's surface structure, producing either microporous or flat films.
  • The resulting PLLA films supported the growth of mesenchymal stem cells and fibroblasts, demonstrated effective drug entrapment and release, making them promising materials for regenerative medicine in treating chronic wounds.
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The depolymerization effect of UV/HO on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied. The structural changes of PSF before and after UV/HO treatment were analyzed, and molecular weight changes during in vitro digestion were determined. Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.

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Formation of peritoneal adhesions is common complication after abdominal and pelvic surgery. They bear a significant health problem with an influence to quality of life and health care expenses. Promising approach for their prevention is using of biodegradable barrier films for physical separation of peritoneal surfaces.

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Article Synopsis
  • Layer-by-Layer assembled polyelectrolyte films utilize negatively charged biopolymers and positively charged polysaccharides to influence cell attachment and behavior on surfaces.
  • The study focused on the properties of these films, particularly those containing chitosan, which exhibited linear growth, smooth surfaces, and viscoelastic characteristics, while films with polyethyleneimine showed increased rigidity and roughness.
  • When tested with mesenchymal stem cells, chitosan films promoted effective cell adhesion and high viability, suggesting that the stiffness and surface properties of these films are crucial for tissue engineering applications.
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