Evaporative light scattering detection based HPLC method for the determination of polysorbate 80 in therapeutic protein formulations.

An evaporative light scattering detection (ELSD) based high-performance liquid chromatography (HPLC) method is developed for the determination of polysorbate 80 (tween 80) in therapeutic protein formulations. The method is simple and overcomes the difficulties associated with specificity and sensitivity. The method is suitable for the quantitation of polysorbate 80 in the usual formulation range (0.

Quantitative analysis of tris(2-carboxyethyl)phosphine by anion-exchange chromatography and evaporative light-scattering detection.

Tris(2-carboxyethyl)phosphine (TCEP) belongs to the trialkylphosphine class of reducing agents that are widely used in research and industry. In this paper, we discuss a sensitive high-performance liquid chromatography (HPLC) method equipped with an evaporative light scattering detector (ELSD) for the determination of TCEP in pharmaceutical samples containing therapeutic protein and stabilizing additives. TCEP was first completely oxidized with hydrogen peroxide to form TCEP oxide (TCEPO).