Proteomics study revealed altered proteome of Dichogaster curgensis upon exposure to fly ash.

Fly ash is toxic and its escalating use as a soil amendment and disposal by dumping into environment is receiving alarming attention due to its impact on environment. Proteomics technology is being used for environmental studies since proteins respond rapidly when an organism is exposed to a toxicant, and hence soil engineers such as earthworms are used as model organisms to assess the toxic effects of soil toxicants. This study adopted proteomics technology and profiled proteome of earthworm Dichogaster curgensis that was exposed to fly ash, with main aim to elucidate fly ash effects on cellular and metabolic pathways.

Biomarker responses in the earthworm, Dichogaster curgensis exposed to fly ash polluted soils.

Earthworms are globally accepted as a model organism in terrestrial ecotoxicology for assessment of environmental pollution. This study evaluated and compared effects of fly ash polluted soils collected from two geographically different thermal power plants on biomarker responses in the earthworm, Dichogaster curgensis. To evaluate relationship between distance sampling and biomarker responses in the earthworm D.

Chemical Treatment as a Pre- requisite for Effective Disposal of Tri Nitro Toluene (TNT).

Present methods for the disposal of old and rejected ammunition carry high risk and are not environment-friendly. Various processes such as wet air oxidation, molten salt oxidation, hydro thermal oxidation, incineration, electrochemical reduction, biodegradation and other methods have limited use for decontamination and are not suitable for disposal of large quantities of explosives. Thus there is dire need to develop alternate method for safe disposal of rejected explosives which will be eco-friendly.

Inter-annual variability of urolithiasis epidemic from semi-arid part of Deccan Volcanic Province, India: climatic and hydrogeochemical perspectives.

Semi-arid Karha basin from Deccan Volcanic Province, India was investigated for inter-annual variability of urolithiasis epidemic. The number of reported urolith patient, weather station data and groundwater quality results was used to assess impact of geoenvironment on urolithiasis. Data of 7081 urolith patient were processed for epidemiological study.

Proteomic profiling and interactome analysis of ER-positive/HER2/neu negative invasive ductal carcinoma of the breast: towards proteomics biomarkers.

Breast cancer, especially ER positive/HER2/neu negative IDC, is the predominant subtype of invasive ductal carcinoma. Although proteomic approaches have been used towards biomarker discovery in clinical breast cancer, ER positive/HER2/neu negative IDC is the least studied subtype. To discover biomarkers, as well as to understand the molecular events associated with disease progression of estrogen receptor positive/HER2/neu negative subtype of invasive ductal carcinoma, differential protein expression profiling was performed by using LC-MS(E) (MS at elevated energy).

Effect of fly ash on biochemical responses and DNA damage in earthworm, Dichogaster curgensis.

Fly ash is receiving alarming attention due to its hazardous nature, widespread usage, and the manner of disposal; leading to environmental deterioration. We carried out bio-monitoring and risk assessment of fly ash in earthworms as a model system. Dichogaster curgensis were allowed to grow in presence or absence of fly ash (0-40%, w/w) for 1, 7, and 14 d.

Analysis of AGE modified proteins and RAGE expression in HER2/neu negative invasive ductal carcinoma.

Cancer is associated with increased glycolysis and carbonyl stress. In view of this, AGE modified proteins were identified from clinical breast cancer tissue using 2DE-immunoblot and mass-spectrometry. These proteins were identified to be serotransferrin, fibrinogen gamma chain, glycerol-3-phosphate dehydrogenase, lactate dehydrogenase, annexin II, prohibitin and peroxiredoxin 6, which have established role in cancer.

Effect of a novel biphenyl compound, VMNS2e on ob/ob mice.

VMNS2e is a novel biphenyl compound, which in previous studies had showed most favourable interactions with the active site of protein tyrosine phosphatase 1B (PTP1B). The effect of acute and chronic treatment of VMNS2e (30mg/kg) was investigated in ob/ob mice. Plasma glucose was measured after acute administration of VMNS2e (30mg/kg) in both lean and ob/ob mice.

Novel biphenyl compound, VMNS2e, ameliorates streptozotocin-induced diabetic nephropathy in rats.

Aim: To study the effect of a new biphenyl synthetic compound showing interactions with the active site of protein tyrosine phosphatase 1B by docking and molecular dynamics, VMNS2e in streptozotocin-induced diabetic nephropathy in rats with various renal function parameters and renal ultrastructure.

Methods: Streptozotocin (55 mg/kg)-induced diabetic rats were orally treated once daily with VMNS2e (30, 60, and 120 mg/kg) for 8 weeks. The body weight and blood glucose levels of the rats were recorded during the study period.

Assessment of air pollution and its effects on the health status of the workers in beam rolling mills factory (Iran National Steel Industrial Group) from Ahvaz-Iran.

Background: Air pollutants of iron- and steel-making operations have historically been an environmental and health hazard. These pollutants include gaseous substances such as sulfur oxide, nitrogen dioxide, and carbon monoxide. The Iran National Steel Industrial Group beam rolling mills factory has two production lines viz.

Aerobic reduction of perchlorate by bacteria isolated in Kerala, South India.

Possibility of perchlorate reduction by microbes raises hope for an eco-friendly mode of degradation of this toxic rocket fuel. This study reports 3 isolates (A1, A2 and A3) capable of molybdenum-independent degradation of perchlorate under aerobic conditions. The rate of degradation was the highest when perchlorate concentration was 17 mM, and then 3.

Investigation of respirable particulate matter pollutants on air-breathing zone workers in the Beam Rolling Mills Factory (Iran National Steel Industrial Group), Ahvaz, Iran.

Workers of iron and steel factories are exposed to a wide range of pollutants depending on the particular process, the materials involved, the effectiveness of monitoring and the control measures. Adverse effects are determined by the physical state and propensities of the pollutant involved, the intensity and duration of the exposure, the extent of pollutant accumulation in the body and the sensitivity of the individual to its effects. The main aim of this study is to assess the levels of the indoor respirable particulate matter (RPM) and to compare the health condition of exposed workers, with nonexposed employees group.

In vitro and in vivo genotoxicity assessment of Cr(VI) using comet assay in earthworm coelomocytes.

Hexavalent chromium is the major terrestrial pollutant released from electroplating industries, tanneries, thermal power plants and sewage. Cr(VI) is reported genotoxicant in vertebrates. Sub-lethal level of Cr(VI) can cause DNA damage.

Clinical and demographic features of infection caused by Acinetobacter species.

Background: Recently, Acinetobacter emerged as an important pathogen and the prevalence of isolation has increased since the last two decades worldwide.

Aims: To determine Acinetobacter incidence, their clinical demography, antibiotyping and speciation.

Settings And Design: A study of the clinical samples submitted to microbiology laboratory of a teaching hospital over a period of 3 years (December 1994 through November 1997).

Alcohol dehydrogenase: a potential new marker for diagnosis of intestinal ischemia using rat as a model.

Aim: Intestinal ischemia (Ii) is an abdominal emergency due to blockade of the superior mesenteric artery resulting in 60-100% mortality if diagnosed late. Changes in several biochemical parameters such as D (-)-lactate, Creatinine kinase isoenzymes and lactate dehydrogenase suggested for early diagnosis, lack specificity and sensitivity. Therefore a biochemical parameter with greater sensitivity needs to be identified.

Multidrug resistant Acinetobacter baumannii isolates from a teaching hospital.

Abstract Acinetobacter baumannii is the most common nosocomial pathogen among all Acinetobacter spp. A. baumannii tend to be resistant to multiple antibiotics, and represent a severe threat in the treatment of hospitalized patients.