Development of High-Resolution DNA Melting Analysis for Simultaneous Detection of Potato Mop-Top Virus and Its Vector, , in Soil.

In this study, a set of duplex reverse transcription PCR (RT-PCR)-mediated high-resolution DNA melting (HRM) analyses for simultaneous detection of potato mop-virus (PMTV) and its protist vector, f. sp. (Sss), was developed.

Identification and Characterization of Lineage A1/D1 from Crops in Manitoba, Canada.

Verticillium stripe in canola ( L.) caused by was first reported in Manitoba in 2014. In this study, crops including canola, mustard () and radish () with visible symptoms of Verticillium stripe were collected from Portage La Prairie, Manitoba, and the pathogens were isolated.

Proliferation of Recombinant PVY Strains in Two Potato-Producing Regions of Canada, and Symptom Expression in 30 Important Potato Varieties with Different PVY Strains.

(PVY) exists as several strains with distinct symptomology and tuber yield effects in different potato varieties. Recently, new recombinant strains have proliferated and dominated local populations around the world. In this study, PVY, PVY, PVY, and PVY strains were tracked across Canada from 2014 to 2017, showing rapid evolution of populations away from the traditionally dominant PVY to recombinants PVY (western Canada) and PVY (eastern Canada).