High-Efficiency Multiplexed Cytosine Base Editors for Natural Product Synthesis in .

is an industrial host with a high fatty acid flux. Even though CRISPR-based tools have accelerated its metabolic engineering, there remains a need to develop tools for rapid multiplexed strain engineering to accelerate the design-build-test-learn cycle. Base editors have the potential to perform high-efficiency multiplexed gene editing because they do not depend upon double-stranded DNA breaks.

Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica.

Yarrowia lipolytica has emerged as a biomanufacturing platform for a variety of industrial applications. It has been demonstrated to be a robust cell factory for the production of renewable chemicals and enzymes for fuel, feed, oleochemical, nutraceutical and pharmaceutical applications. Metabolic engineering of this non-conventional yeast started through conventional molecular genetic engineering tools; however, recent advances in gene/genome editing systems, such as CRISPR-Cas9, transposons, and TALENs, has greatly expanded the applications of synthetic biology, metabolic engineering and functional genomics of Y.

Heterologous biosynthesis of natural product naringenin by co-culture engineering.

Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals. In this study, co-cultures were developed for heterologous biosynthesis of the natural product naringenin. The co-cultures were composed of two independent strains dedicated to functional expression of different portions of the biosynthetic pathway, respectively.