Objectives: In-vitro studies were performed on dentin of extracted human molars to investigate the effectiveness of 9.3 μm CO laser irradiation to occlude dentinal tubules. The observed occlusion of dentinal tubules with the irradiation was compared with application of three reagents: 2% Sodium Fluoride gel, an aqueous solution of hydroxyapatite nanoparticles and an equal mix of the two.
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